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Plasmid#167555PurposeBackbone plasmid to pRMA154DepositorTypeEmpty backboneUseBroad host range mobilisableTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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pBBR-Cas9ΔyqhD-HA500
Plasmid#220369PurposeA single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440DepositorInsertsSpCas9
sgRNA for yqhD in P. putida KT2440
UseCRISPR; Broad host rangeTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFTG5
Plasmid#149487PurposeBroad-Host range expression vector, RSF1010 origin, TetR repressor, PTetA promoter, mRFP.DepositorInsertTetR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTetAAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAH01
Plasmid#128162PurposeIncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP220
Plasmid#185921PurposeBroad host range IncP promoter probe using lacZDepositorTypeEmpty backboneUseIncpTagsLacZExpressionBacterialMutationPromoterNoneAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBRG2
Plasmid#149474PurposeBroad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertRhaS-RhaR-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLxR5
Plasmid#149482PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLxVi5
Plasmid#167516PurposeBroad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.DepositorInsertLuxR-Violacein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBNG2
Plasmid#149473PurposeBroad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertNahR(AM)-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKCiR5
Plasmid#149462PurposeBroad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBCiG2
Plasmid#149470PurposeBroad-Host range expression vector, pBBR origin, CinR(AM) activator, PCin promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertCinR(AM)-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPSalTTCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLxR3
Plasmid#167518PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLxR4
Plasmid#167517PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Carbenicillin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWLxR5
Plasmid#149490PurposeBroad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFAR5
Plasmid#149478PurposeBroad-Host range expression vector, RSF1010 origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertAraC-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLxR5
Plasmid#149465PurposeBroad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWVR5
Plasmid#167509PurposeBroad-Host range expression vector, pSa origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertVanRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKCyR5
Plasmid#149463PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLxR5
Plasmid#167504PurposeBroad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only