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pT2K-p53DN-T2A-copGFP

Plasmid

#109229

Purpose

For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

dominant negative p53 (Trp53 Mouse)

Use

Tags

copGFP

Expression

Mammalian

Mutation

dominant negative p53

Promoter

CAGGS

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_p53(R280K)_V5

Plasmid

#136540

Purpose

Lentiviral expression vector for an inducible p53(R280K)-V5

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53(R280K) (TP53 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

V5

Expression

Mammalian

Mutation

p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

Promoter

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2_p53(R280K)_V5

Plasmid

#136525

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53(R280K) (TP53 Human)

Use

Entry vector for gateway cloning

Tags

V5

Expression

Mutation

p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pShuttle-PR.CC9

Plasmid

#192932

Purpose

pShuttle.CC9 encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAd-PR.Cre

Plasmid

#192936

Purpose

Adenoviral expression vector encoding sgRNAs targeting Trp53 and Rb1 and Cre

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Inserts

Trp53 (Trp53 Mouse)
Rb1 (Rb1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pShuttle-PRL.Cre

Plasmid

#192934

Purpose

pShuttle.Cre encoding sgRNAs targeting Trp53, Rb1 and Rbl2 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1, Rbl2 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pShuttle-PR.Cre

Plasmid

#192933

Purpose

pShuttle.Cre encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAd-PR.CC9

Plasmid

#192935

Purpose

Adenoviral expression vector encoding sgRNAs targeting Trp53 and Rb1, Cre and Cas9

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Inserts

Trp53 (Trp53 Mouse)
Rb1 (Rb1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAd-PRL.Cre

Plasmid

#192937

Purpose

Adenoviral expression vector encoding sgRNAs targeting Trp53, Rb1 and Rbl2 and Cre

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Inserts

Rb1 (Rb1 Mouse)
Trp53 (Trp53 Mouse)
Rbl2 (Rbl2 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pYFP-NEAT1pr_v1-RT

Plasmid

#97087

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.

Depositor

Archa Fox

Insert

nuclear paraspeckle assembly transcript 1 (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Expression

Mutation

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Pig3 Promoter

Plasmid

#16489

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

PIG3 promoter (TP53I3 Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pDest17_E3 ubiquitin-protein ligase (Topors) LCD (379-1045)

Plasmid

#235005

Purpose

Bacterial expression of N-terminally 6His tagged E3 ubiquitin-protein ligase (Topors) LCD

Depositor

Tanja Mittag

Insert

E3 ubiquitin-protein ligase (Topors) (TOPORS Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBK-CMV Pig3

Plasmid

#16496

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig3 (TP53I3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
Pig3 Delta Promoter

Plasmid

#16490

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

PIG3 promoter (TP53I3 Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pCEP4 Pig4

Plasmid

#16498

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig4 (SAA1 Human)

Use

Tags

HA tag variant - YAYDVPDYA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pBK-CMV Pig12

Plasmid

#16506

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig12 (PTGES Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pCRII Pig5

Plasmid

#16499

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig5 (ANO9 Human)

Use

Tags

Expression

Bacterial

Mutation

Partial cDNA clone

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pCRII Pig9

Plasmid

#16503

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig9 (MAD1L1 Human)

Use

Tags

Expression

Bacterial

Mutation

Partial cDNA clone

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
N-Myc-53BP1 WT pLPC-Puro

Plasmid

#19836

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Oct 19. ():.

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pBK-CMV Pig10

Plasmid

#16504

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig10 (ENC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2010

Availability

Academic Institutions and Nonprofits only