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pMAL-C2X dAmp/Kana+-2AFP

Plasmid

#154358

Purpose

Bacterial expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal F423R/V435R/W427R

Plasmid

#47351

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Ha

Expression

Mammalian

Mutation

F423R/V435R/W427R

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pLenti CMV/TO SV40 small + Large T (w612-1)

Plasmid

#22298

Depositor

Eric Campeau

Insert

SV40 small + Large T antigen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kdm1a-exon minimal CMV pCDNA5

Plasmid

#212054

Purpose

LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a-exon (Kdm1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense

Plasmid

#46834

Purpose

Express cGFP transcript ending in the wildtype MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pDF0352 HvsCas7-11 with C-term Nes mammalian expression

Plasmid

#172516

Purpose

Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

C-term Nes HvsCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

Plasmid

#186835

Purpose

Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 513/515/516 to Alanine

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

Plasmid

#131731

Purpose

Transposon based transgenesis

Depositor

Rene Maehr

Article

Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

Insert

SV40MinPromoter

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Chtop-exon minimal CMV pCDNA5

Plasmid

#212080

Purpose

LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop-exon (Chtop Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC

Plasmid

#148443

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-Malat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Chx10 (3kb proximal 5' region)-GFP-IRES-AP

Plasmid

#18815

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Chx10 (3kb proximal 5' region)-GFP-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pET21 Catalytic Domain of MALT1 Catalytic Mutant

Plasmid

#48971

Purpose

Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566; Catalytic Mutant C464A

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pCDH-hMALAT1 deletion 11 PARP1 binding sites

Plasmid

#159327

Purpose

Mammalian cell expression of human MALAT1 with deleted PARP1 binding sites. Contains CopGFP marker.

Depositor

Jianjun Zhao

Insert

MALAT1 (MALAT1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion 11 PARP1 binding sites

Promoter

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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HLH Bmal Pas 1-465-VenC I317D

Plasmid

#47367

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, I317D

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMAL-c5e-Rubicon RH N821K, T911R, K916R

Plasmid

#216707

Purpose

Bacterial protein expression of Rubicon RH domain mutant

Depositor

James Hurley

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

Rubicon (RUBCN Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

N821K, T911R, K916R

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mta1+exon minimal CMV pCDNA5

Plasmid

#212070

Purpose

LR vector for integration of Mta1+exon(12nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mta1+exon(12nt) (Mta1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mta1-exon minimal CMV pCDNA5

Plasmid

#212071

Purpose

LR vector for integration of Mta1-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mta1+exon(12nt) (Mta1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gatad2a+exon minimal CMV pCDNA5

Plasmid

#212050

Purpose

LR vector for integration of Gatad2a+exon(75nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gatad2a+exon(75nt) (Gatad2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gatad2a-exon minimal CMV pCDNA5

Plasmid

#212051

Purpose

LR vector for integration of Gatad2a-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gatad2a-exon (Gatad2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8-exon minimal CMV pCDNA5

Plasmid

#212078

Purpose

LR vector for integration of Zmynd8-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8-exon (Zmynd8 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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