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Plasmid#123336PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.DepositorInsertCerulean3-cpCerulean3-FLARE-AKAR
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR
Plasmid#123335PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertCerulean3-Cerulean3-FLARE-AKAR
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)
Plasmid#123330PurposeNegative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.DepositorInsertVenus-cpVenus-FLARE-AKAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75240PurposeCRISPR/Cas9 plasmid against human IkarosDepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75241PurposeCRISPR/Cas9 plasmid against human IkarosDepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR
Plasmid#123338PurposeMitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertDAKAP-Venus-cpVenus-FLARE-AKAR
UseTagsN-terminal 30 amino acids from DAKAP1, Venus, and…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)
Plasmid#86421PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequenceDepositorInsertCDC42pr-KAR2SS-GFP1-10
UseTagsGFP1-10ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR
Plasmid#123337PurposePlasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertLyn-Venus-cpVenus-FLARE-AKAR
UseTagsN-terminal targeting sequence from Lyn kinase, Ve…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA E171Q GFP
Plasmid#32127DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationE171QPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA K108A GFP
Plasmid#32125DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP, HDEL, and Kar2ExpressionYeastMutationK108APromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE E171Q GFP
Plasmid#32128DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, E171QPromoterAvailable sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Oskar 241-387 (DR) (HK74)
Plasmid#206462PurposeOskar 240-387 expression in Schneider cells for imagingDepositorInsertOskar 240-387 (osk Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
H3-pAc5.1 OST4 mCherry Oskar 139-606
Plasmid#206494PurposeOskar 139-606 expression for Schneider cell imagingDepositorInsertOskar 139-606
UseTagsOST4 mcherryExpressionInsectMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE E171Q HDEL
Plasmid#32124DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, E171QPromoterAvailable sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE K108A GFP
Plasmid#32126DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, K108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA E171Q HDEL
Plasmid#32123DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationK108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE K108A HDEL
Plasmid#32122DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, K108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA K108A HDEL
Plasmid#32121DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationK108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75243PurposeCRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)DepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75242PurposeCRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)DepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only