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Plasmid#115202PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MFSD9_STOP
Plasmid#161060PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertMFSD9 (MFSD9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS2 (CK2alpha', CK2beta)
Plasmid#27093DepositorUseTetracycline-regulated expressionTagsHA and MycExpressionMammalianMutationPromoterbidirectional tet-responsive promoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S293A
Plasmid#115204PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291A/S293A
Plasmid#115205PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2
Plasmid#115199PurposeLentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291A
Plasmid#115203PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DCAF4L1:1-396
Plasmid#211238PurposeMammalian expression of full-length human DCAF4L1. N-terminal HiBiT tag.DepositorInsertDCAF4L1:M1-S396 (DCAF4L1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DCAF4L1:1-396
Plasmid#211312PurposeMammalian expression of full-length human DCAF4L1. C-terminal HiBiT tag.DepositorInsertDCAF4L1:M1-S396 (DCAF4L1 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZP10 Snail
Plasmid#16931DepositorInsertsnail (snai1a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
PCRII-zSynB
Plasmid#20122DepositorInsertzSynB (sncga Zebrafish)
UseIn-situTagsExpressionMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
SOCS2A-c016
Plasmid#53689Purposebacterial expression plasmidDepositorInsertSOCS2A
UseTagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
KBB609
Plasmid#185110PurposePom152 N-terminus in pEX-N-His for bacterial expression and purificationDepositorInsertPOM152
UseTags6HisExpressionBacterialMutationPom152 N-term-6HisPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AK5A-c001
Plasmid#53674Purposebacterial expression plasmidDepositorInsertAK5A
UseTagsHis6-TEVExpressionBacterialMutationR156M, Bacterial expression for structure determi…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-get1
Plasmid#192722PurposeGateway entry vector encoding zebrafish get1DepositorInsertget1 (wrb Zebrafish)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZNF609.1.0-gDNA
Plasmid#132447PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF609 (ZNF609 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZNF319.1.0-gDNA
Plasmid#132438PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF319 (ZNF319 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
KBB610
Plasmid#185111PurposePom152 full repeat domain in pEX-N-His for bacterial expression and purificationDepositorInsertPOM152
UseTags6HisExpressionBacterialMutationPom152 Repeats-6HisPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-zgc:110006
Plasmid#192737PurposeGateway entry vector encoding zebrafish zgc:110006DepositorInsertzgc:110006 (zgc:110006 Zebrafish)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB611
Plasmid#185112PurposePom152 central repeats 4 and 5 in pEX-N-His for bacterial expression and purificationDepositorInsertPOM152
UseTags6HisExpressionBacterialMutationPom152 repeats 4&5-6HisPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only