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Plasmid#214445PurposeExpresses eSpCas9 and gRNA targeting Pansio-1 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Olônne
Plasmid#214447PurposeExpresses eSpCas9 and gRNA targeting Olônne-18 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA10.53-Clover
Plasmid#214450PurposeBxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.DepositorInsertmClover
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Keppel
Plasmid#214446PurposeExpresses eSpCas9 and gRNA targeting Keppel-19 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-JAM-A
Plasmid#112965PurposeTo express feline Jam-A transiently in cell lines that do not naturally express the receptor.DepositorInsertJunctional Adhesion Molecule type A (JAM-A) (F11R Felis catus)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGG426_UV5
Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNA0304
Plasmid#165612PurposeVector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)DepositorInsertSNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
UseCRISPRTagsExpressionYeastMutationNotI site replaced the CAN1 spacer in parent vect…PromoterSNR52Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hGRM1-RFP
Plasmid#22920DepositorInserthuman receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mA93-RFP
Plasmid#22918DepositorInsertmouse riken gene A930038C07Rik promoter driving DsRedExpress (A930038C07Rik Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
OR5-consensus
Plasmid#208062PurposeConsensus OR5 engineered from human OR5 family membersDepositorInsertOR5-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR51-consensus
Plasmid#208065PurposeConsensus OR51 engineered from human OR51 family membersDepositorInsertOR51-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR6-consensus
Plasmid#208063PurposeConsensus OR6 engineered from human OR6 family membersDepositorInsertOR6-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR8-consensus
Plasmid#208064PurposeConsensus OR8 engineered from human OR8 family membersDepositorInsertOR8-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR1-consensus
Plasmid#208059PurposeConsensus OR1 engineered from human OR1 family membersDepositorInsertOR1-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR2-consensus
Plasmid#208060PurposeConsensus OR2 engineered from human OR2 family membersDepositorInsertOR2-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR10-consensus
Plasmid#208058PurposeConsensus OR10 engineered from human OR10 family membersDepositorInsertOR10-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR4-consensus
Plasmid#208061PurposeConsensus OR4 engineered from human OR4 family membersDepositorInsertOR4-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR52-consensus
Plasmid#208080PurposeConsensus OR52 engineered from human OR52 family membersDepositorInsertOR52-consensus
UseTagsRhodopsin tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG438
Plasmid#165486PurposeVector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAGDepositorInsertMammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
UseCRISPRTags3x FLAG and SV40 NLSExpressionMammalianMutationD1135V, S1136R, D1332K, and R1333G mutations in S…PromoterCMV and T7Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-7Zf-wild
Plasmid#23160DepositorInsertRibonuclease assay sub1
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only