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pLKO.1 - TRC mTagBFP2

Plasmid

#191566

Purpose

Expresses an shRNA and mTagBFP2

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for shRNA

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-mTagBFP2

Plasmid

#99374

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a and hU6

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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NES-mTagBFP2-P4Mx1

Plasmid

#108142

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTBL3478 PB-PEmax-mTagBFP2

Plasmid

#226664

Purpose

PiggyBac cargo vector encoding PEmax and mTagBFP2

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Inserts

PEmax
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG and UBC

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-COX2-EBFP2

Plasmid

#184720

Purpose

Exogenous COX2

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

COX2-EBFP2 (PTGS2 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLX - T7pro - EBFP

Plasmid

#138615

Purpose

Express EBFP with an upstream T7 promoter in a lentiviral backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EBFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRVdG-4BFP2-5postmGRASP

Plasmid

#52498

Purpose

Expresses mTagBFP2 and postsynaptic mGRASP component

Depositor

Ian Wickersham

Inserts

mTagBFP2
postsynaptic mGRASP

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab11a

Plasmid

#79805

Purpose

Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Use

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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BFP-Sec61 beta

Plasmid

#49154

Purpose

expression of Sec61 beta fused to BFP, used as a general ER marker

Depositor

Gia Voeltz

Article

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Insert

Sec61 beta (SEC61B Human)

Use

Tags

BFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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lenti-NES-mTagBFP2

Plasmid

#216129

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-MAPKKnucelarExportSignal-mTagBFP2-WPRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A

Plasmid

#102585

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from SFFV promoter

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ H2B-mTagBFP2

Plasmid

#99267

Purpose

Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Philipp Keller

Insert

mTagBFP2-3XHA

Use

Tags

H2B

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_blast-tagBFP-lox5171

Plasmid

#162072

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMT Shark-TagBFP

Plasmid

#126658

Purpose

Inducible expression of Shark-TagBFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-sgCDKN1B#2 BFP

Plasmid

#60905

Purpose

A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-Cas9-2A-BFP

Plasmid

#164662

Purpose

Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

Cas9
BFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSSV_LTR

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pKH151_PB_flag-LC3B_wt_IRES-BFP

Plasmid

#214976

Purpose

Flag tagged LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-BFP

Plasmid

#153426

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

mTagBFP2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX TRE 3G BFP

Plasmid

#128070

Purpose

Lentiviral expression of Tet inducible TagBFP

Depositor

Oskar Laur

Insert

TagBFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

no

Promoter

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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