-
Plasmid#105180Purposesynthetic promoter HIF1A drives mKate2 expressionDepositorInsertS(HIF1A)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-WPRE
Plasmid#51505PurposeCan be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoterDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
nontarget S. pyogenes scaffold CARGO array
Plasmid#191319PurposeContains an array of nontargeting guide RNAsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614G
Plasmid#158075PurposeExpresses SARS-CoV-2 spike protein with a D614G mutationDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationD614GPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-s ONE-GO
Plasmid#204327PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-s ONE-GO (GNAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. pyogenes scaffold CARGO array
Plasmid#191316PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Skylan-S-(GGGGS)x3-β-Actin
Plasmid#128938Purposefluorescnece reporter of β-Actin with Skylan-S. Express in mammalian cellsDepositorInsertSkylan-S
UseTagsβ-actinExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614
Plasmid#158074PurposeExpresses SARS-CoV-2 spike proteinDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-VPR-S-L1-dCjCas9 MiniCAFE
Plasmid#169910PurposeExpression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.DepositorInsertMiniCAFE
UseCRISPRTagsExpressionMammalianMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S (D614G)
Plasmid#215838PurposeExpresses SARS-CoV-2 Spike protein in mammalian cellsDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.2-RBD
Plasmid#184830PurposeMammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
UseTags6X His tagExpressionMammalianMutationSARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-EGFP-WPRE
Plasmid#51504PurposeCan be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoterDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EOS-S(4+)-EGFP
Plasmid#21317PurposeLentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFPDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralTagsExpressionMammalianMutationPromoterEOS-S(4+) promoter regionAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-D614 (C-flag)
Plasmid#156420PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.1-RBD
Plasmid#184831PurposeMammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
UseTags6X His tagExpressionMammalianMutationSARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-G614 (C-flag)
Plasmid#156421PurposeMammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationD614GPromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.617.2.v1
Plasmid#182575PurposeMammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950NDepositorInsertSpike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-D614 (NC-flag)
Plasmid#156418PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorInsertSARS-CoV-2 S protein (S SARS CoV2)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG DEPTOR (13×S/T->A)
Plasmid#21702DepositorInsertDEPTOR 13xA (DEPTOR Human)
UseTagsFLAGExpressionMammalianMutationNonphosphorylatable mutant. 13xA. Also has N204S …PromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-NLS-NmCas9-HA-NLS(s)
Plasmid#47867PurposeThis plasmid encode NmCas9 with two NLS and a HA tagDepositorInsertNmCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only