-
Plasmid#127527PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)Bxb1
Plasmid#127528PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pEF-GFP(rc)phiC31
Plasmid#127510PurposePlasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S(rc)2_4_5-GFP
Plasmid#127520PurposePlasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.DepositorInsertCaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXspuro-GFP
Plasmid#74203PurposeRetroviral control vector containing GFP insertDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-X0
Plasmid#115565PurposeControl vector expressing GFP alone without any consensus repeatsDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterial and YeastMutationPromoterADH1Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 shCavin1KDR-EGFP
Plasmid#187254PurposeLentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)DepositorInsertShRNA for Cavin1 (Cavin1 Mouse)
UseLentiviralTagsEGFPExpressionMutationPromoterU6, 5'LTRAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE
Plasmid#202198PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotorDepositorInsertstChrimsonR, PdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX
Plasmid#112762Purposeexpress GFP-4EBP1-Rheb15(CAAX mutation)DepositorInsertFlag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
UseLentiviralTagsRheb15(CAAX mutant)ExpressionMutationCAAX mutation to prevent lipid localizationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-EGFP
Plasmid#50457PurposeDouble floxed EGFP under the control of human synapsin promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, and AAV8InsertEGFP
UseAAVTagsN/AExpressionMutationPromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pccGFPGpA
Plasmid#73651PurposeFluorescent reporter for transmembrane protein dimerization positive control.DepositorInsertglycophorin A
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCYC1m_yeGFP
Plasmid#64389Purposeencodes yeast enhanced GFP under control of minimal pCYC1 promoterDepositorInsertyeast enhanced GFP
UseTagsExpressionYeastMutationPromoterpCYC1Available sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGFP150K
Plasmid#193313PurposepAS control plasmid for sfGFP expressionDepositorInsertsuper folder GFP
UseTagsExpressionMammalianMutation150K in sfGFPPromoterEF1Available sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
Plasmid#110145PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(K68A,K69A,F70A,F71A) in pcDNA3.1
Plasmid#42189DepositorInsertGamma 3(K68A,K69A,F70A,F71A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationK68A,K69A,F70A,F71APromoterCMVAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)
Plasmid#208863PurposeStably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagyDepositorInsertNAP1 (AZI2 Human)
UseTagsFKBP-GFPExpressionMammalianMutationS37K/A44E (disrupts NDP52 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)
Plasmid#208864PurposeStably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagyDepositorInsertNAP1 (AZI2 Human)
UseTagsFKBP-GFPExpressionMammalianMutationI11S/L12S (disrupts FIP200 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)
Plasmid#208865PurposeStably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagyDepositorInsertNAP1 (AZI2 Human)
UseTagsFKBP-GFPExpressionMammalianMutationL226Q/L233Q (disrupts TBK1 binding)PromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)
Plasmid#110144PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-d2eGFP-cxcr4
Plasmid#21967DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-eGFP_IDG-K
Plasmid#135265PurposeGateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-eGFP
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG05-pGlnA-deGFP
Plasmid#102462PurposeExpresses deGFP fluorescent protein in E. coli. The expression is controlled by a pGlnA promoter.DepositorArticleInsertdeGFP
UseTagsExpressionBacterialMutationPromoterpGlnAAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5-FRT-V5-DEST-GFP-Kif26b
Plasmid#102862PurposeExpresses GFP-Kif26b in mammalian cellsDepositorInsertMouse Kif26b (Kif26b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human 3' HP1a AID GFP PuroR
Plasmid#127906PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a GeneDepositorInsertHP1 (CBX5 Human, Mustard Weed)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationInserting GFP AID 2A Puro into mouse 3' Hp1aPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-AICD-IRES-hrGFP
Plasmid#107548Purposehigh transduction efficiency AAV-mediated synapsin promoter-dependent expression of AICD (last 50 amino acids of APP) and IRES-mediated co-expression of hrGFPDepositorInsertAICD (APP Human)
UseAAVTagsExpressionMutationNonePromoterhuman synapsinAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-AICD-NLS-IRES-hrGFP
Plasmid#107543PurposeAAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFPDepositorInsertAICD-NLS (APP Human)
UseAAVTagsExpressionMutationNonePromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-AICD-NES-IRES-hrGFP
Plasmid#107544PurposeAAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFPDepositorInsertAICD-NES (APP Human)
UseAAVTagsExpressionMutationNonePromoterCMVAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MYC-GFP-IRES-mCherry
Plasmid#231232PurposeMYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MCRS1-GFP-IRES-mCherry
Plasmid#231231PurposeMCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorInsertMCRS1 (MCRS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn GFP-Cre
Plasmid#175381Purposeexpresses GFP-Cre under the control of a human synapsin promoterDepositorInsertGFP-Cre recombinase
UseAAVTagsCre is fused to GFP. GFP is fused to additional n…ExpressionMutationPromoterhuman synapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pP3-GFP
Plasmid#53436PurposeControls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genomeDepositorInsertP3 promoter
UseSynthetic BiologyTagsnoneExpressionBacterialMutationnonePromoterP3Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSiren_EGFP-shLuc
Plasmid#122287PurposeKnockdown controlDepositorInsertKnockdown shRNA for Luc
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-WT
Plasmid#212119PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLTE EGFP
Plasmid#67044PurposeCell-free expression controlDepositorInsertEGFP
UseCell-free expressionTagsMycExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
Plasmid#218916PurposeLentiviral construct expressing msfGFP and nuclear mCherry proteins.DepositorInsertEF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(414)/GFP-AUT7(414)
Plasmid#49424PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationPromoterATG8Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pccGFPG83I
Plasmid#73650PurposeFluorescent reporter for transmembrane protein dimerization negative control.DepositorInsertglycophorin A- G83I
UseTagsExpressionBacterialMutationglycine 83 to isoleucinePromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmGFP
Plasmid#117926PurposeControl plasmid expressing myc-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-GFP-V5_IDG-K
Plasmid#135242PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-GFP_IDG-K
Plasmid#135275PurposeGateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-GFP
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pGK-nAChRb2(E61C)-IRES-GFP
Plasmid#164777Purposecysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligandDepositorInsertnAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
UseLentiviralTagsExpressionMammalianMutationE61CPromoterpGKAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-CMV-nAChRb2(E61C)-IRES-GFP
Plasmid#164779Purposecysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligandDepositorInsertnAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)
UseTagsExpressionMammalianMutationE61CPromoterCMVAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP
Plasmid#50465PurposeEGFP under the control of human synapsin promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial sizeInsertEGFP
UseAAVTagsN/AExpressionMutationPromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CBA::EGFP-P2A-tC3
Plasmid#129476PurposeAAV2 transfer plasmid for truncated C3 with EGFP under control of the CBA promoterDepositorInserttruncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-KASH2ext
Plasmid#187018PurposeControl for inducible disruption of LINC ComplexDepositorInsertKASH2ext
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
TATA-GFP
Plasmid#110833Purposenegative controlDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP
Plasmid#179840Purposedonor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR and TALEN; Donor plasmidTagschloride-conducting ChR (ChloC) and eGFPExpressionMammalianMutationPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-eGFP NLS (no drug resistant gene)
Plasmid#140032PurposeTunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
cTRE-LSL-GFP-IRES-Cas9-sgCR8
Plasmid#135668PurposeIntroduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchangeDepositorInsertsgCR8
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-GFP-V5-TurboID_IDG-K
Plasmid#135241PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5-TurboID
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only