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Showing: 281 - 300 of 2410 results

  3. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid
    #208973
    Purpose
    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208973-plasmid-map-sequence-id-415830

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  4. pOttc1501 - pscAAV CMV-IE hCDNF

    Plasmid
    #188538
    Purpose
    An AAV packaging vector that expresses hCDNF under control of the CMV-IE promoter.
    Depositor
    Brandon Harvey
    Insert
    hCDNF (CDNF Human)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV-IE
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188538-plasmid-map-sequence-id-370038

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  5. pLenti-CMV-PtenC124S-Puro

    Plasmid
    #135677
    Purpose
    Constitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    C124S
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135677-plasmid-map-sequence-id-327588

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  6. pLenti-CMV-PtenG129E-Puro

    Plasmid
    #135678
    Purpose
    Constitutive expression (cMV promoter) of G129E mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    G129E
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135678-plasmid-map-sequence-id-327597

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  7. pLenti-CMV-PtenK13E-Puro

    Plasmid
    #135680
    Purpose
    Constitutive expression (cMV promoter) of K13E mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    K13E
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135680-plasmid-map-sequence-id-327598

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  8. pLenti-CMV-PtenY138L-Puro

    Plasmid
    #135679
    Purpose
    Constitutive expression (cMV promoter) of Y138L mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Y138L
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135679-plasmid-map-sequence-id-327594

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  10. pJEP12-AAV-H1/TO-L-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82704
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82704-plasmid-map-sequence-id-158051

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  11. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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  12. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82705
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    82705-plasmid-map-sequence-id-170450

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  13. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82702
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82702-plasmid-map-sequence-id-163793

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  14. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82701
    Purpose
    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82701-plasmid-map-sequence-id-157303

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  15. pGL4_10xUAS-CMVmin-BC0443-luc2

    Plasmid
    #227131
    Purpose
    Barcoded assay, UAS sensor for split TEV assays; barcode BC0443
    Depositor
    Michael Wehr
    Insert
    10x clustered UAS element linked to CMV minimal promoter driving barcode 0443 and a luciferase reporter gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227131-plasmid-map-sequence-id-454371

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  16. pGL4_CRE-CMVmin-BC0481-luc2

    Plasmid
    #227132
    Purpose
    Barcoded assay, cAMP/Ca2+ sensor; barcode BC0481
    Depositor
    Michael Wehr
    Insert
    6x clustered CRE element linked to CMV minimal promoter driving barcode 0481 and a luciferase reporter gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227132-plasmid-map-sequence-id-454370

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  17. pGL4_10xUAS-CMVmin-BC0411-luc2

    Plasmid
    #227122
    Purpose
    Barcoded assay, UAS sensor for split TEV assays; barcode BC0411
    Depositor
    Michael Wehr
    Insert
    10x clustered UAS element linked to CMV minimal promoter driving barcode 0411 and a luciferase reporter gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    227122-plasmid-map-sequence-id-452575

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  18. pGL4_10xUAS-CMVmin-BC0405-luc2

    Plasmid
    #227118
    Purpose
    Barcoded assay, UAS sensor for split TEV assays; barcode BC0405
    Depositor
    Michael Wehr
    Insert
    10x clustered UAS element linked to CMV minimal promoter driving barcode 0405 and a luciferase reporter gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    227118-plasmid-map-sequence-id-452574

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  19. pGL4_10xUAS-CMVmin-BC0408-luc2

    Plasmid
    #227120
    Purpose
    Barcoded assay, UAS sensor for split TEV assays; barcode BC0408
    Depositor
    Michael Wehr
    Insert
    10x clustered UAS element linked to CMV minimal promoter driving barcode 0408 and a luciferase reporter gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    227120-plasmid-map-sequence-id-452573

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  20. pGL4_10xUAS-CMVmin-BC0441-luc2

    Plasmid
    #227129
    Purpose
    Barcoded assay, UAS sensor for split TEV assays; barcode BC0441
    Depositor
    Michael Wehr
    Insert
    10x clustered UAS element linked to CMV minimal promoter driving barcode 0441 and a luciferase reporter gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    227129-plasmid-map-sequence-id-452572

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Showing: 281 - 300 of 2410 results