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Plasmid#159078PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0713
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0621:Dendra
Plasmid#159070PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0621
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0415:Dendra
Plasmid#159064PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0415
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0301:Dendra
Plasmid#159050PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0301
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0225:Dendra
Plasmid#159043PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0225
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0710:Dendra
Plasmid#159020PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0710
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0384:Dendra
Plasmid#159013PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0384
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0229:Dendra
Plasmid#159005PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0229
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0024:Dendra
Plasmid#158997PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0024
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ZNF251
Plasmid#101451PurposeDonor Vector containing ZNF251 transcription factor, part of the Human TFome CollectionDepositorInsertZNF251 (ZNF251 Human)
UseGateway shuttling vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-Runx1+23
Plasmid#69602PurposeRunx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloningDepositorInsertsUse5' gateway entry vectorTagsExpressionMutationPromoterC57/BL6 mouse Runx1 +23 intronic enhancer and C57…Available sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424TagsExpressionMutationThe conserved GDD in the NS5 polymerase gene muta…PromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS10
Plasmid#107924PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ281 (MbCas12a)
Plasmid#138113PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized MbCas12a without promoterDepositorInsertMbCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS11
Plasmid#107925PurposeamdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS-mEmerald-VIM
Plasmid#62849PurposeDonor vector for gene editing at human VIM locusDepositorInsertmEmerald-VIM (VIM Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-STU
Plasmid#138110PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized LbCas12a without promoter and terminatorDepositorInsertLbCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
Plasmid#221893PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertTAF15 (TAF15 Human)
UseTags6xHis-MBP-PreScission and TEV-mGFPExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC-G418-YR
Plasmid#61767PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).DepositorInsertsnptII gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBGE3.0
Plasmid#165988PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
neomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only