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Showing: 2961 - 2980 of 6179 results

  3. pWPT-/UAA-mEGFP-IRES-mCherry

    Plasmid
    #49234
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49234-plasmid-map-sequence-id-73657

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  4. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid
    #138211
    Purpose
    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKAsub-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138211-plasmid-map-sequence-id-307413

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  13. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid
    #230996
    Purpose
    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.
    Depositor
    Michael Rape
    Article
    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:
    Type
    Empty backbone
    Use
    Tags
    AcGFP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    230996-plasmid-map-sequence-id-457945

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  20. pAAV-anti-mCherry(LaM8) PAGER(TF)

    Plasmid
    #229999
    Purpose
    Expresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229999-plasmid-map-sequence-id-457197

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Showing: 2961 - 2980 of 6179 results