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Plasmid#90530Purpose3rd generation lentiviral gRNA plasmid targeting human ANLNDepositorInsertANLN (Guide Designation A1.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TUBB F2.4 gRNA
Plasmid#90925Purpose3rd generation lentiviral gRNA plasmid targeting human TUBBDepositorInsertTUBB (Guide Designation F2.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVMG0173_Cq-Actin5c-Cas9
Plasmid#169345PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0173_Cq-Actin5c-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ009808Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9GG
Plasmid#131009PurposeBackbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.DepositorInsertsdirect repeat of SpCas9
promoter PJ23119
mRFP expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterBba_R0040 TetRAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-TdT-guide1-PGK-Puro
Plasmid#102903PurposeEBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.DepositorInsertTdT-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_MYOD1
Plasmid#64138PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1_MYOD1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133D2.0
Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-T3-CjCAS9-pA
Plasmid#131543PurposeExpresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCjCas9
UseCRISPRTagsFLAG-SV40NLS, SV40NLS, and polyAExpressionMammalianMutationHuman codon-optimizedPromoterCAG, T3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVMG0212_Cq-nanos-Cas9
Plasmid#169348PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0212_Cq-nanos-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ011551Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0213_Cq-vasa-Cas9
Plasmid#169347PurposeExpression of Cas9 in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0213_Cq-vasa-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ009286Available sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
DKC1 F1.3 gRNA
Plasmid#90652Purpose3rd generation lentiviral gRNA plasmid targeting human DKC1DepositorInsertDKC1 (Guide Designation F1.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
NUMA1 H11.2 gRNA
Plasmid#90808Purpose3rd generation lentiviral gRNA plasmid targeting human NUMA1DepositorInsertNUMA1 (Guide Designation H11.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
CBX3 A6.5 gRNA
Plasmid#90569Purpose3rd generation lentiviral gRNA plasmid targeting human CBX3DepositorInsertCBX3 (Guide Designation A6.5)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
UNG D12.3 gRNA
Plasmid#90937Purpose3rd generation lentiviral gRNA plasmid targeting human UNGDepositorInsertUNG (Guide Designation D12.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
PRC1 G2.2 gRNA
Plasmid#90857Purpose3rd generation lentiviral gRNA plasmid targeting human PRC1DepositorInsertPRC1 (Guide Designation G2.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PRC1 G1.2 gRNA
Plasmid#90856Purpose3rd generation lentiviral gRNA plasmid targeting human PRC1DepositorInsertPRC1 (Guide Designation G1.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
NUMA1 H12.2 gRNA
Plasmid#90809Purpose3rd generation lentiviral gRNA plasmid targeting human NUMA1DepositorInsertNUMA1 (Guide Designation H12.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_RXRB_2
Plasmid#104051PurposeEncodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFPDepositorInsertRXRB gRNA (RXRB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only