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Plasmid#227352PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin P37L (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyADO
Plasmid#226248PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyODepositorInsertsHis6_ApyA
ApyD
ApyO
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyAO
Plasmid#226242PurposeProduction of ApyA peptide modified by the cytochrome P450 enzyme ApyODepositorInsertsHis6_ApyA
ApyO
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyAD
Plasmid#226241PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyDDepositorInsertsHis6_ApyA
ApyD
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast CG1358
Plasmid#218541PurposeDrosophila CG1358 cDNA (human codon optimized)DepositorInsertCG1358 (HisT Fly)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
RoL10X_pET29b
Plasmid#215427PurposeExpression construct for RoL10X gene, codon optimized for expression in E. coliDepositorInsertRoL10X
UseTagsExpressionBacterialMutationV209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…PromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB-SCY_pET29b
Plasmid#215415PurposeExpression construct for NylB-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB-SCY
UseTagsExpressionBacterialMutationR187S/F264C/D370YPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
M-NylC_pET28a
Plasmid#215409PurposeExpression construct for M-NylC gene, codon optimized for expression in E. coliDepositorInsertM-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA
Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-AncSZ
Plasmid#214235PurposeBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylationDepositorInsertSyk kinase (SYK Human)
UseTags6xHisExpressionBacterialMutationSequence gained through ancestral reconstruction …PromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOA-Leu-FvPPT1
Plasmid#179923PurposeExpression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertFvPPT1
UseTagsExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7 (2-180)
Plasmid#190066PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEVExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7-Strep (2-180)
Plasmid#190067PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEV and StrepExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUSSTOP
Plasmid#202053PurposeE. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHELDepositorInsertAtWUS E. Coli Codon Optimized (WUS Mustard Weed)
UseTags6xHis and GFP11ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUS
Plasmid#193865PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.DepositorInsertAtWUS E. Coli Codon Optimized
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)
Plasmid#180029PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0001
Plasmid#185625PurposeMoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoterDepositorInsertSpCas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)_co_L-HicDH
Plasmid#185453PurposeCDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).DepositorInsertL-HicDH from Lactobacillus confusus DSM 20196
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB203
Plasmid#169114PurposeTESEC expression vector for mEGFP-Alr fusion constructDepositorInsertmEGFP-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpBADAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only