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Plasmid#62382PurposeExpresses beta actin with both a PAGFP and mRFP tag.DepositorInsertbeta actin (ACTB Human)
UseTagsPAGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF145 pAcGFP1 SOD1G93A
Plasmid#26406DepositorInsertSOD1 (SOD1 Human)
UseTagsGFPExpressionMammalianMutationG93APromoterAvailable sinceNov. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
DsRed-Rab5 DN
Plasmid#13051DepositorInsertRAB5A (Canis familiaris) (RAB5A Canis familiaris)
UseTagsDsRed2 and HAExpressionMammalianMutationasparagine 3 changed to serine (neutral); aspara…PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
GFP-mouse vinculin full length (889)
Plasmid#67935PurposeMammalian expression of mouse vinculin with GFP fusionDepositorInsertVinculin (Vcl Mouse)
UseTagsEGFPExpressionMammalianMutationL159P in vinculinPromoterCMVAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-5ptaseOCRL
Plasmid#66836PurposeExpression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.DepositorInsert5ptaseOCRL (OCRL Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5B-Rigor
Plasmid#172204PurposeExpresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5B (Kif5b Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-T31N (DN)
Plasmid#83423PurposeMouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidue 31 from T to N (T31N: ACG -> AAC)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-5ptaseOCRL(D523G)
Plasmid#66837PurposeExpression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.DepositorInsert5ptaseOCRL (OCRL Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationD523GPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2GPI
Plasmid#203759PurposeEncodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.DepositorInsertCD58 c-term (CD58 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
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cyto-CFP-FRBx5
Plasmid#103776Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertcyto-CFP-FRBx5
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA2w/b
Plasmid#47586DepositorInsertPMCA2w/b (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Tubulin-C-18
Plasmid#54292PurposeLocalization: Microtubules, Excitation: 487, Emission: 509DepositorInsertTubulin (TUBA1B Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mcherry p62 delta UBA silent
Plasmid#187984Purposemcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationLacking UBA domain (aa389-434) but it has the sil…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-PKI nls
Plasmid#118301PurposeExpression of PKIaDepositorInsertPKI nls (aa 2-36)
UseTagsFlag and GFPExpressionMammalianMutationinserting 2xnlsPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2FP
Plasmid#203757PurposeEncodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.DepositorInsertNRas c-term (NRAS Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hGABARAP
Plasmid#87871PurposeExpress a EGFP-human GABARAP in mammalian cellsDepositorInsertGABARAP (GABARAP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
LD-RA
Plasmid#209863PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddRFP A
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
YF-Cdc42(CA)
Plasmid#20152DepositorInsertYFP-FKBP-Cdc42(CA) (CDC42 Human)
UseTagsExpressionMammalianMutationCdc42H was inserted into the multiple cloning sit…PromoterAvailable sinceMarch 26, 2009AvailabilityAcademic Institutions and Nonprofits only