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pCRII-Topo Foxp2 in situ probe

Plasmid

#45620

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Foxp2 in situ probe (Foxp2 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp#1709-2142 of AF339106

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pBT241_(pCA-ATG-intron-tTA2)

Plasmid

#36875

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tTA2

Use

Tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pSLQ1653_sgSOD1

Plasmid

#63711

Purpose

Express sgSOD1 in mammalian cells of human.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

sgSOD1, CAG promoter, mCherry, puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pATM (pKD-G11)

Plasmid

#62690

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419/tdTomato

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTBL3342 PB-PEmax-puro

Plasmid

#226674

Purpose

PiggyBac cargo vector encoding PEmax and puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

PEmax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_Divergent Donor

Plasmid

#229784

Purpose

Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Convergent

Plasmid

#229790

Purpose

Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_mScarlet_Divergent

Plasmid

#229791

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

TRE and CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-HM4Di-YFP

Plasmid

#209193

Purpose

Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

Depositor

Larry Zweifel

Article

Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

Insert

Hm4Di

Use

AAV

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh10.1 GFP

Plasmid

#209102

Purpose

GFP expressing scramble of shRNA targeting Cdh10

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh10.1

Use

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

Plasmid

#210736

Purpose

Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

SaSp D10A Cas9
Merry sgCAG

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Mutation

D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

Promoter

H1 and eCMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

Plasmid

#210735

Purpose

Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

SaSp D10A Cas9
Gollum sgCAG

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Mutation

D10A, N terminal Sa Cas9, C-terminal Sp Cas9

Promoter

H1 and eCMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PB-ReNL

Plasmid

#123138

Purpose

Piggybac transposon plasmid for live animal optical imaging of Red-enhanced nanolantern

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Red-enhanced Nanolantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJM597 ZF(2/6)x3 mKate2 in TUPV1

Plasmid

#161531

Purpose

Inducible expression of mKate2 under the ZF(2/6)x3 promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

ZF(2/6)x3

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MYMH 40

Plasmid

#138034

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-7GC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG CAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 21

Plasmid

#138025

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-4GC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA CAG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pPickles-2.31

Plasmid

#92336

Purpose

FRET-based biosensor for the measurement of BCR-ABL activity

Depositor

Yusuke Ohba

Article

Mizutani et al Clin Cancer Res. 2010 Aug 1;16(15):3964-

Insert

Pickles-2.31

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pATN (pKD-G12)

Plasmid

#62691

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP419/amiR-eGFP123/tdTomato

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pATL (pKD-G10)

Plasmid

#62689

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP419/amiR-eGFP123

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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