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Plasmid#77960Purpose3rd generation lentiviral gRNA plasmid targeting human BMPR1ADepositorInsertBMPR1A (BMPR1A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-CAGGS-ERAS
Plasmid#52416PurposeLentiviral vector encoding constitutive expression of human ERAS cDNADepositorInsertERAS (ERAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-Lam
Plasmid#133899PurposeNegative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWHDepositorInsertLMNA (Lam) (LMNA Human)
UseTagsExpressionYeastMutationPromoterT7Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJ199
Plasmid#162672PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
GNE gRNA (BRDN0001145959)
Plasmid#77832Purpose3rd generation lentiviral gRNA plasmid targeting human GNEDepositorInsertGNE (GNE Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GNE gRNA (BRDN0001145858)
Plasmid#77830Purpose3rd generation lentiviral gRNA plasmid targeting human GNEDepositorInsertGNE (GNE Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-S155,172A
Plasmid#74380PurposeLentiviral expression vector containing flag tagged S155,172A MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseLentiviralTagsFlagExpressionMammalianMutationSer 155 Ala, Ser 172 Ala (Numbering based on MFF …PromoterAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) FITM2
Plasmid#107504PurposeExpresses FITM2, puromycin resistantDepositorInsertFITM2 (codon optimized)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
GNE gRNA (BRDN0001149214)
Plasmid#77831Purpose3rd generation lentiviral gRNA plasmid targeting human GNEDepositorInsertGNE (GNE Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIB Flag MFF S155,172A
Plasmid#74387PurposeRetroviral expression vector containing Flag tagged S155,172A MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseRetroviralTagsFlagExpressionMammalianMutationSer 155 Ala, Ser 172 Ala (Numbering based on MFF …PromoterCMVAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.blast-Flag-MFF-S155,172E
Plasmid#74443PurposeLentiviral expression vector containing flag tagged S155,172E MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseLentiviralTagsFlagExpressionMammalianMutationSer 155 Glu, Ser 172 Glu (Numbering based on MFF …PromoterAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Anti-DNAH7 [N250/21.4R]
Plasmid#164158PurposeMammalian Expression Plasmid of anti-DNAH7 (Human). Derived from hybridoma N250/21.4.DepositorInsertanti-DNAH7 (Homo sapiens) recombinant mouse monoclonal antibody (DNAH7 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
GNE gRNA (BRDN0001149237)
Plasmid#77833Purpose3rd generation lentiviral gRNA plasmid targeting human GNEDepositorInsertGNE (GNE Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCJ203
Plasmid#162678PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)DepositorInsertPutative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
UseTagsHisExpressionBacterialMutationdeltaF2-A75; codon optimized for expression in E.…PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL S113D
Plasmid#107161PurposeFor mammalian expression of Flag-hPKL S113DDepositorInsertFlag-hPKL S113D (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationSerine 113 mutated to aspartatePromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPKL S113A
Plasmid#107160PurposeFor mammalian expression of Flag-hPKL S113ADepositorInsertFlag-hPKL S113A (PKLR Human)
UseRetroviralTagsN-term FlagExpressionMammalianMutationSerine 113 mutated to alaninePromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAP3K5 gRNA (BRDN0001148435)
Plasmid#76654Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K5DepositorInsertMAP3K5 (MAP3K5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-lox-ERAS
Plasmid#52417PurposeDoxycycline inducible lentiviral vector of human ERAS cDNA with excisable insertDepositorInsertERAS (ERAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQCXIB Flag MFF S155A
Plasmid#74385PurposeRetroviral expression vector containing Flag tagged S155A MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseRetroviralTagsFlagExpressionMammalianMutationSer 155 Ala (numbering based on MFF isoform 1)PromoterCMVAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIB Flag MFF S172A
Plasmid#74386PurposeRetroviral expression vector containing Flag tagged S172A MFF isoform 5 mutantDepositorInsertFlag-MFF (MFF Human)
UseRetroviralTagsFlagExpressionMammalianMutationSer 172 Ala (numbering based on MFF isoform 1)PromoterCMVAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only