-
Plasmid#200899PurposeExpresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.DepositorInsertPseCas7, PseCas8, PseCas6, PseTniQ
UseTagsNLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)ExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mTurquoise2
Plasmid#98817PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorArticleInsert3xNLS
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL4A
Plasmid#19951DepositorInsertCUL4A (cullin 4A) (CUL4A Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGG-B1
Plasmid#78504PurposeTrans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsidDepositorInsertsAAV2 Rep
AAV-B1 capsid
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFP/Cre
Plasmid#49056Purposeadeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.DepositorInsertCre
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP, Myc, and NLSExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNTI827
Plasmid#216635PurposeCal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression and lentivirus productionDepositorArticleInsertCal-ID-ERM
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
HIVGKO
Plasmid#112234PurposeHIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1aDepositorInsertcsGFP-EF1a-mKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHyVec11
Plasmid#34794DepositorTypeEmpty backboneUseHydra expressionTagsIntergenic region between Hydra replication factoā¦ExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
Plasmid#50946PurposeAn empty gRNA expression vectorDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tau (wt) -VC
Plasmid#87369Purposeexpresses hTau fused to the C-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsc-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisExpressionMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_KLF4
Plasmid#26815DepositorInsert5'UTR-KLF4-3'UTR (KLF4 Human)
UseTagsExpressionMammalianMutationStarts from second ATGPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressioā¦PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCN-mBFP
Plasmid#63847PurposeEncodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.DepositorInsertmBFP
UseTagsExpressionBacterialMutationPromoter and RBS was swapped. His-tag was removedā¦PromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b_Bst-LF
Plasmid#153313PurposeBacterial expression of the Bst Large FragmentDepositorInsertBst Large Fragment
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TetO-dCas9-D3A
Plasmid#78254PurposeExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RR
Plasmid#108860PurposeLbCpf1 Gateway entry plasmid with G548R and K611R mutationsDepositorInsertLbCpf1-RR (LbCpf1 with G548R and K611R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R and K611R, Cpf1 is rice codon optimizedPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX puro TRIM21-GFP
Plasmid#116941PurposeExpression of TRIM21-GFP fusion proteinDepositorInsertTRIM21 - GFP (TRIM21 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304-ARNT
Plasmid#203578PurposeExpresses wild-type ARNT in mammalian cells.DepositorInsertARNT (ARNT Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALEN-mROSA26 ELD
Plasmid#60026PurposeExpression of a TALEN protein specific for mouse ROSA26 locus.DepositorInsertTALEN-ROSA26
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYCipi
Plasmid#53279PurposeContains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE
Plasmid#162374PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4
Plasmid#27002DepositorInsertNEDD4 (NEDD4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-foldon-GFP- PCP
Plasmid#194503PurposeExpression foldon-GFP-PCP protein for imaging of nonrepetitive DNADepositorInsertfoldon-GFP-PCP
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1Ī± core promoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-N1
Plasmid#54843PurposeLocalization: N1 Cloning Vector, Excitation: 434, Emission: 474DepositorTypeEmpty backboneUseTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3xFLAG SARS-CoV2 RdRp.co
Plasmid#154008PurposeExpression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cellsDepositorInsertCodon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
UseTags3xFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-pEF1a-EBFP-tTAn
Plasmid#172122PurposeConstitutive expression of EBFP and rtTA-InteinNDepositorInsertEBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZS2oxySp-GFP-proD-oxyR
Plasmid#78224Purposesynthetic gene circuitDepositorInsertsynthetic gene circuit
UseTagsExpressionMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSICO-eGFP-TNPO3
Plasmid#167590PurposeExpresses human TNPO3 fused to eGFPDepositorInserteGFP-TNPO3 (TNPO3 Human)
UseLentiviralTagseGFPExpressionMutationWTPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
EB3-tdTomato
Plasmid#50708Purposeexpresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTsDepositorInsertEB3 (MAPRE3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-T7-KPNA4
Plasmid#26680DepositorInsertKaryopherin Alpha 4 (KPNA4 Human)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBPZpGAL4DBDUw
Plasmid#26233DepositorInsertZip-GAL4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
TALEN-mROSA26 KKR
Plasmid#60025PurposeExpression of a TALEN protein specific for mouse ROSA26 locus.DepositorInsertTALEN-ROSA26
UseTALENTagsExpressionMammalianMutationPromoterSV40Available sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type ā¦Promotermaize Ubiquitin-1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-mRuby3
Plasmid#74252PurposeMammalian expression of tandem fusion of mClover3-mRuby3DepositorInsertmClover3-mRuby3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-mouse-ATP6-1397C-AD(V28R)
Plasmid#212955PurposeExpresses TALED_Right-mouse-ATP6-1397C-AD(V28R) in mammalian cellsDepositorInsertRight-mouse-ATP6-1397C-AD(V28R) (ATP6 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-mouse-ATP6-1397N.
Plasmid#212951PurposeExpresses TALED_Left-mouse-ATP6-1397N in mammalian cellsDepositorInsertTALED_Left-mouse-ATP6-1397N (ATP6 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
UseTagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-tā¦PromoterT7Available sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-RVRR
Plasmid#160327PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoterDepositorInsertMb2Cas12a RVRR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN563R, K569V, N573R and K625RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-mNG-Ec-Kbp-D9N
Plasmid#178011PurposeBacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)DepositorInsertmNG-Ec-Kbp-D9N
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
TB:5xNF- ĪŗĪ²_RE:MiniP
Plasmid#133878Purpose5 copies of the NF-ĪŗĪ² DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3-MAP4-MTBD
Plasmid#171498PurposeT7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNADepositorInsertMAP4-MTBD (Map4 Mouse)
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
Plasmid#71294PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGNSTM glycosylation motif, HA, Lamp2 signal peptiā¦ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF3007
Plasmid#144483PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD1 (SMAD1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pREXC3GH
Plasmid#47077PurposeFX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneUseLactococcus lactis expressionTags3C protease site, GFP, and HisExpressionMutationPromoterNIS (nisin)Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLC-217
Plasmid#73163PurposeTemplate plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.DepositorInsertPrhaB-relE (relE E. coli (bacteria))
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastMutationPromoterGPDAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL-3F-BirA
Plasmid#220147PurposeExpresses VHL-BirA for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationcodon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-I997A
Plasmid#111832PurposeVinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vectorDepositorInsertVinculinTS (I997A) (VCL Chicken)
UseLentiviralTagsExpressionMutationchanged vinculin isoleucine 997 to alaninePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only