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Plasmid#152843PurposeMammalian expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ubiquitin K48C
Plasmid#86588PurposeBacterial expression of Ubiquitin K48C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; K48CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
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6975 pPROEX-HTb-SIS1
Plasmid#1233DepositorInsertsis1 (SIS1 Budding Yeast)
UseTagshis tag (tev cleavable)ExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET22HT-PP7delFG
Plasmid#31863DepositorInsertPP7delFG Coat Protein
UseTagsHis6-tag and TEV siteExpressionMutationdeleted FG loopPromoterAvailable sinceAug. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMCSG17-NleL
Plasmid#66716PurposeExpresses NleL (170-782) in E. coliDepositorInsertNleL (NEWENTRY )
UseTagsGST-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pNIC-TolB-NHSTIIT-GG
Plasmid#215792PurposeCloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
FGD5
Plasmid#28109PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertFGD5:HPC09X-H08:C212499 (FGD5 Human)
UseTagsHis-tag with TEV siteExpressionBacterialMutationcontains amino acid residues 889-1304.PromoterAvailable sinceMarch 21, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ubiquitin K63C
Plasmid#86589PurposeBacterial expression of Ubiquitin K63C for cross linking studiesDepositorInsertUbiquitin (UBC Human)
UseTagsHis6-MBPExpressionBacterialMutationTEV site; K63CPromoterTacAvailable sinceFeb. 24, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pGAT3
Plasmid#112589PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusionsDepositorTypeEmpty backboneUseTags6His-GSTExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
6974 pPROEX-HTb-SSB1
Plasmid#1232DepositorInsertssb1 (SSB1 Budding Yeast)
UseTagshis tag (tev cleavable)ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
6C1A (MBD2)
Plasmid#119958PurposeBacterial expression for structure determinationDepositorInsertMBD2 (MBD2 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ANO10 (TMEM16KA-c001)
Plasmid#110267PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMEM16K (ANO10 Human)
UseTagsTEV-His10-FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
6972 pPROEX-HTb-SSA1
Plasmid#1231DepositorInsertSsa1 (SSA1 Budding Yeast)
UseTagshis tag (tev cleavable)ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133902
Plasmid#152159PurposeMammalian expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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JMJD1CA-c089
Plasmid#79644PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertJMJD1C (JMJD1C Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only