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pDY380

Plasmid

#182962

Purpose

Amp-resistant, low copy (p15A ori), E. coli 5'ptsI transcriptionally fused with GFP.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Insert

ptsI

Use

Synthetic Biology

Tags

GFP

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDY390

Plasmid

#182963

Purpose

Amp-resistant, low copy (p15A ori), E. coli ptsI transcriptionally fused with GFP.

Depositor

Shelley Copley

Article

Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

Insert

ptsI

Use

Synthetic Biology

Tags

GFP

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LLP765_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IF_lacZ

Plasmid

#192409

Purpose

To act as a 1-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB602-TP-mCherry-UnaG

Plasmid

#203483

Purpose

For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused mCherry-UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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BPTI-[5,55]Gly14Ile38

Plasmid

#195059

Purpose

Thermal stability of BPTI

Depositor

Yutaka Kuroda

Article

Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

Insert

bovine pancreatic trypsin inhibitor

Use

Tags

His tag and trpΔLE leader sequences

Expression

Bacterial

Mutation

Changed Alanine residues at position of 14 and 38…

Promoter

T7 promotor

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-PAH1-3HA

Plasmid

#184761

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

PAH1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-CDS1-3HA

Plasmid

#184760

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

CDS1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-DGK1-3HA

Plasmid

#184759

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Use

Tags

3HA

Expression

Yeast

Mutation

Promoter

DGK1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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OM14-2EGFP-UG74-ter

Plasmid

#184758

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

OM14 (OM14 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2EGFP-ter-NAT

Plasmid

#184764

Purpose

Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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IRE1-2EGFP-ter-kanMX4

Plasmid

#184766

Purpose

Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

IRE1 (IRE1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EMC1-2EGFP-ter-NAT

Plasmid

#184763

Purpose

Marker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

EMC1 (EMC1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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LLP744_L2pV1_OR_B3RT_FRT_Rluc_1IF_lacZ

Plasmid

#192388

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Use

Tags

2Katushka

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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p8271 LentiCRISPR v2 hygro sgSIGIRR-4

Plasmid

#193980

Purpose

Expression of spCas9 and sgRNA targeting SIGIRR

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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LLP761_L2pV1_OR-OCS_s35S-Cre

Plasmid

#192420

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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LLP673_L2_pV01_1I_VloxP-OCS-Rluc_TCTP_VCre_lacZ

Plasmid

#192425

Purpose

To act as the on state for testing of the alt VCre recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::VloxP-OCS-VloxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP749_L2pV1_AND_B3RT_FRT_Rluc_1IF_lacZ

Plasmid

#192393

Purpose

To act as a 1-input state of the AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP748_L2pV1_AND_B3RT_FRT_Rluc_0I_lacZ

Plasmid

#192392

Purpose

To act as the 0-input state of the AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP671_L2_pV01_1I_SloxM1-OCS-Rluc_TCTP_SCre_lacZ

Plasmid

#192423

Purpose

To act as the on state for testing of the alt SCre recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::SloxM1-OCS-SloxM1::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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