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Plasmid#170279PurposeExpression of mutant HIV-2 RT p66 reverse transcriptaseDepositorInsertHIV-2 RT p66 (mutant)
UseTagsExpressionBacterialMutationPromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-CUL1-3'UTR
Plasmid#97156PurposeFor dual luciferase report assayDepositorInsertCUL1 3'UTR (CUL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGV3340
Plasmid#170278PurposeExpression of mutant HIV-2 RT p51 reverse transcriptaseDepositorInsertHIV-2 RT p51 (mutant)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-SART1
Plasmid#38087DepositorInsertSART1 squamous cell carcinoma antigen recognized by T cells (SART1 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Sce)
Plasmid#45576PurposeCodes for the SSA-TLR with the target site for I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E3CY56
Plasmid#103075PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX317-RBFOX1
Plasmid#115442PurposeConstitutive lentiviral expressionDepositorInsertRBFOX1 (RBFOX1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Hygro-Myc-hAgo2
Plasmid#60234PurposeExpress HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible mannerDepositorInsertArgonaute2 (AGO2 Human)
UseLentiviralTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP(iL)-Grx1(human)
Plasmid#65002PurposeYeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1DepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsroGFP(iL)ExpressionYeastMutationPromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)
Plasmid#99229PurposeMammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.DepositorInsertpr-mEos2
UseTagsLifeactExpressionMammalianMutationA69T for primed conversion applicationsPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SFFV-NFE2L18ND-Puro
Plasmid#181918PurposeLentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutation8 putative N-glycoyslation sites converted from a…PromoterSFFVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAGEdeltaCyto
Plasmid#71436Purposemammalian expression of human RAGE cytoplasmic domain mutantDepositorInsertRAGE (AGER Human)
UseTagsExpressionMammalianMutationdelta 367-404PromoterCMVAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) mouse C/EBP gamma
Plasmid#12560DepositorInsertCCAAT enhancer binding protein gamma (Cebpg Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro C-Raf 22W
Plasmid#12593DepositorInsertN-term truncated mut of Raf-1 (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutation22W, N-terminally truncatedPromoterAvailable sinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pIMVAd39T
Plasmid#98303PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.DepositorInsertpdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-NPC1(D786N)-FLAG
Plasmid#164975PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationD786N, insertion L aa15 (please see depositor co…PromoterEF1aAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL236
Plasmid#173184PurposeCRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 (EZH2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL214
Plasmid#171031PurposeCRISPR plasmids encodes Cas9 and a sgRNA targeting EZH2 exon 2 - intron 2 junction (sequence: GCAGACGAGCTGATGAAGTAA)DepositorInsertEZH2 (EZH2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only