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Plasmid#66085Purposeco-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORFDepositorInsertsgRNA for APa4-2
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCeSR beta deltaN
Plasmid#13728DepositorInsertSignal recognition particle receptor β subunit (R186.3 Nematode)
UseTags6xHisExpressionBacterialMutationexpresses residues 38-240 of C. elegans SRβ with …PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHCMM1
Plasmid#167452PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoterDepositorInsertPTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pHCMM2
Plasmid#167453PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoterDepositorInsertPSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterPSEC63Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pHCMM3
Plasmid#167454PurposeA control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]DepositorInsertPSEC63_yeGFP_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterPSEC63Available sinceAvailabilityAcademic Institutions and Nonprofits only -
T7-Elongin C-pcDNA3
Plasmid#19998DepositorInsertElongin C (ELOC Human)
UseTagsT7-tagExpressionMammalianMutationPromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-EGFP
Plasmid#153163PurposeMouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromotermSncgAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A
Plasmid#100146PurposeExpresses CPT1ADepositorInsertCPT1A (CPT1A Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-RIGI-Apex- T2A-EGFP
Plasmid#167288PurposeLentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.DepositorInsertDDX58 (RIGI Human)
UseLentiviralTagsAPEX2 and EGFPExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATG9A-EGFP
Plasmid#210367PurposeExpresses ATG9A fused with EGFP in mammalian cellsDepositorInsertATG9A (ATG9A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_NRF2
Plasmid#159129Purposeexpresses NRF2 in mammalian cellsDepositorInsertNRF2 (NFE2L2 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6HuR
Plasmid#135943PurposeExpresses HuR in E. coliDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab7 DN
Plasmid#12660DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsGFP and HAExpressionMammalianMutationT22NPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE-2XFLAG,2XHA-CyclinF-puro
Plasmid#32976DepositorInsertCYCLIN F (CCNF Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoter5'LTRAvailable sinceFeb. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre sgAmotl2 v3
Plasmid#158602Purposelenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2DepositorInsertAmotl2 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
DsRed-Rab5 DN
Plasmid#13051DepositorInsertRAB5A (Canis familiaris) (RAB5A Canis familiaris)
UseTagsDsRed2 and HAExpressionMammalianMutationasparagine 3 changed to serine (neutral); aspara…PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-LGC-53
Plasmid#86399PurposeMammalian expression of codon-optimized dopamine receptor LGC-53DepositorInsertLGC-53 (lgc-53 Nematode, Synthetic)
UseTagsExpressionMammalianMutationCodon optimized for mammalian expression; Signal …PromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p7140 pHAGE-P-CMVt-N-HA-GAW-EME1
Plasmid#100150PurposeExpresses EME1DepositorInsertEME1 (EME1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GSDMD-Myc
Plasmid#201166PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D (Gsdmd Mouse)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only