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Plasmid#49686DepositorInsertECF30_83
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a13-87
Plasmid#49816Purposeexpression plasmid for SRAP(13-87) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-87 (SRA1 Human)
UseTagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa11_3726
Plasmid#49662DepositorInsertECF11_3726
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a13-215
Plasmid#49817Purposeexpression vector for SRAP(13-215) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-215 (SRA1 Human)
UseTagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-miniCGBE1
Plasmid#198548PurposeExpresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-miniCGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-miniCGBE1
Plasmid#198538PurposeExpresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-miniCGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRRH
Plasmid#198540PurposeExpresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRRH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRTH
Plasmid#198541PurposeExpresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRTH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH
Plasmid#198542PurposeExpresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin Ubl P37L
Plasmid#227366PurposeFor bacterial expression of mutated parkin Ubl.DepositorInsertParkin 1-76 P37L (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin K161N
Plasmid#227365PurposeFor bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.DepositorInsertParkin K161N (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin delta 101-109
Plasmid#227364PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin delta 101-109 (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Resi…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin D106A
Plasmid#227362PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin D106A (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin V105K
Plasmid#227361PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin V105K (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin T103A
Plasmid#227360PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin T103A (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin L102K
Plasmid#227359PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin L102K (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin M458L
Plasmid#227356PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin M458L (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin R256C
Plasmid#227355PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin R256C (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin R234Q
Plasmid#227354PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin R234Q (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin V224A
Plasmid#227353PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin V224A (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only