Addgene

Showing: 2941 - 2960 of 11749 results

Results per page:

Sort results by:

PB-SRT-Puro_BC5

Plasmid

#193147

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Use

Tags

Expression

Mammalian

Mutation

Barcode: GTCA

Promoter

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-SRT-Puro_BC3

Plasmid

#193145

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Use

Tags

Expression

Mammalian

Mutation

Barcode: CCAA

Promoter

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-SRT-Puro_BC2

Plasmid

#193144

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Use

Tags

Expression

Mammalian

Mutation

Barcode: CTGA

Promoter

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-SRT-Puro_BC1

Plasmid

#193143

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Use

Tags

Expression

Mammalian

Mutation

Barcode: CACA

Promoter

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414-PCY1:S495A

Plasmid

#98151

Purpose

Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:S495A

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed serine 495 to alanine

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414-PCY1:W603A

Plasmid

#98153

Purpose

Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:W603A

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tryptophan 603 to alanine

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414-PCY1:H695Q

Plasmid

#98154

Purpose

Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695Q

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to glutamine

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414-PCY1:H695A

Plasmid

#98155

Purpose

Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695A

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to alanine

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ414-PCY1:Y696G

Plasmid

#98156

Purpose

Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:Y696G

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tyrosine 696 to glycine

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RRRAA-5-mut-pHG165c

Plasmid

#170113

Purpose

Constitutively expressed, mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

RRRAA-5-MUT

Use

Tags

Expression

Bacterial

Mutation

5A14L, W116Q, E178K, S184T, S237L

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Mm_Plk5(D168A)

Plasmid

#136318

Purpose

Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

D168A

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Hu_Plk5(W207F)

Plasmid

#136320

Purpose

Mammalian expression of human Plk5 W207F with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Use

Tags

Expression

Mammalian

Mutation

W207F

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14b-dabAB2 D353A

Plasmid

#133016

Purpose

pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Use

Tags

6xHis, StrepII tag, and sfGFP

Expression

Bacterial

Mutation

D353A

Promoter

t7

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14b-dabAB2 C351A

Plasmid

#133014

Purpose

pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Use

Tags

6xHis, StrepII tag, and sfGFP

Expression

Bacterial

Mutation

C351A

Promoter

t7

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DYNLL2

Plasmid

#132531

Purpose

Human LC8-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL2 (DYNLL2 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DYNLT3

Plasmid

#132532

Purpose

Human TCTEX-3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLT3 (DYNLT3 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DYNLRB2

Plasmid

#132534

Purpose

Human Rbl-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB2 (DYNLRB2 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)

Plasmid

#112803

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Jurkiewicz et al Biochim Biophys Acta. 2017 May;1864(5):7

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Use

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 554_557 deleted/PDZ

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p3 FLAG_CMV14/OCTN2 (delta14_22)

Plasmid

#112800

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Use

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 14_22 deleted

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

Plasmid

#110159

Purpose

Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge