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pCW57.1 _3xHA-Ago2_CATmut

Plasmid

#73540

Purpose

Inducible lentiviral expression of Ago2_CATmut

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Catalytic mutant PIWI domain D670A

Promoter

TRE promoter, Tet ON

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-S155,172D

Plasmid

#74442

Purpose

Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Asp, Ser 172 Asp (Numbering based on MFF …

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
FUW-Mbd3

Plasmid

#52356

Purpose

Lentiviral expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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TFORF1440

Plasmid

#143808

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ARID4A (ARID4A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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mIFP-LaminA-C-18

Plasmid

#56226

Purpose

Localization: Nuclear Envelope, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LaminA (LMNA Human)

Use

Tags

mIFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1/FGFR2-S252W(Apert) myc

Plasmid

#33249

Depositor

Alka Mansukhani, Claudio Basilico

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Mutation S252W in FGFR2 found in Apert syndrome. …

Promoter

CMV

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-Puro-FH-AGO2-5xE

Plasmid

#91993

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001145474)

Plasmid

#77347

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-5xA

Plasmid

#91979

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001146786)

Plasmid

#77346

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-5xA

Plasmid

#91992

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-5xE

Plasmid

#91981

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND2

Plasmid

#172629

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2-FDM3

Plasmid

#72210

Purpose

Mammalian expression of aa 227-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-226

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
TFORF3316

Plasmid

#144792

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MED21 (MED21 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2-FDM1

Plasmid

#72208

Purpose

Mammalian expression of aa 1-372 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 373-860

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM2

Plasmid

#72209

Purpose

Mammalian expression of aa 1-517 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 518-860

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM4

Plasmid

#72211

Purpose

Mammalian expression of aa 373-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-372

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
mIFP12-LaminA-C-18

Plasmid

#56256

Purpose

Localization: Nuclear Envelope, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LaminA (LMNA Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCMV5-Flag-Ago2-FDM5

Plasmid

#72212

Purpose

Mammalian expression of aa 518-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-517

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only