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Plasmid#211163PurposeMammalian expression of full-length human CDC20. C-terminal HiBiT tag.DepositorInsertCDC20:M1-R499 (CDC20 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB513B-1/TRE-hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBPγ-EF1α-Puro
Plasmid#199550PurposePiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1α-hHNF3β-hCEBPα-hCEBPβ-hCEBP-γ) under control of the TRE/PCMVmin promoterDepositorUsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterTRE+CMVmin promoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_CDC20:161-477
Plasmid#210943PurposeInsect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.DepositorInsertCDC20:S161-P477 (CDC20 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_CDC20:161-P477
Plasmid#210888PurposeInsect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertCDC20:S161-P477 (CDC20 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc_hsHAUScomplex_FL
Plasmid#202203PurposeBaculoviral transfer vector to co-express 8 subunits of human augmin complexDepositorInsertsHAUS1 (HAUS1 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS3 (HAUS3 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS2 (HAUS2 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS6 (HAUS6 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS7 (HAUS7 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS4 (HAUS4 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS5 (HAUS5 codon-optimized form for expression in Trichoplusia ni cells, Human)
HAUS8 (HAUS8 codon-optimized form for expression in Trichoplusia ni cells, Human)
UseTags-mGFP-TEV-TwinStrep and His-tag (6x)ExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
JGI_018101
Plasmid#158920PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_028327376_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_017953
Plasmid#158881PurposeHydrocarbon Structures for Fuels and ChemicalsDepositorInsertWP_063765283_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Quas:GFPpA;Betacrystallin:CFP
Plasmid#81181PurposeReplaces #61376. QUAS driving GFP with beta crystalline CFP eye markerDepositorInsertQUAS:GFP;beta crys:CFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTMR6A
Plasmid#42483PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMTMR6A
UseTagsHis6ExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
TNKS2A
Plasmid#42491PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTNKS2A
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1B-EGFP-DmRigor-mortis_AA
Plasmid#148203PurposeInsect Expression of DmRigor-mortisDepositorInsertDmRigor-mortis (rig Fly)
UseTagsExpressionInsectMutation25 silent and 12 non-silent mutations compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmRigor-mortis_AA
Plasmid#148218PurposeInsect Expression of DmRigor-mortisDepositorInsertDmRigor-mortis (rig Fly)
UseTagsExpressionInsectMutation25 silent and 12 non-silent mutations compared to…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G
Plasmid#146359PurposeInsect Expression of DmDCP2-PBDepositorInsertDmDCP2-PB (DCP2 Fly)
UseTagsExpressionInsectMutationContains an in-frame insertion that adds a Q afte…PromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZNF584-donor
Plasmid#112342PurposeCRISPR donor plasmid to tag human transcription factor ZNF584 with GFPDepositorInsertZNF584 homology arms flanking EGFP-IRES-Neo cassette (ZNF584 Human)
UseCRISPRTagsExpressionMutationhg38:19:58417078:T>C, rs7257872, rs3764535PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X36
Plasmid#59260PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt82-ex4-5
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pZNF778-donor
Plasmid#112379PurposeCRISPR donor plasmid to tag human transcription factor ZNF778 with GFPDepositorInsertZNF778 homology arms flanking EGFP-IRES-Neo cassette (ZNF778 Human)
UseCRISPRTagsExpressionMutationrs4785627,rs28415940,rs9921361,rs28638280,rs60437…PromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-IFT88-N-18
Plasmid#54125PurposeLocalization: Cilium, Excitation: 487, Emission: 509DepositorInsertIFT88 (Ift88 Mouse)
UseTagsmEmeraldExpressionMammalianMutationN241D in IFT88PromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits