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Plasmid#40444DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; V1922D, V2048SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
LRRK2_1867_2161_F1908Y_L2115A
Plasmid#40445DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; F1908Y, L2115APromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
LRRK2_1867_2161_F1908A_V2048S
Plasmid#40446DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; F1908A, V2048SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
LRRK2_1867_2161_I2131S
Plasmid#40448DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; I2131SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
LRRK2_1867_2161_L1936Y_I2131S
Plasmid#40449DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; L1936Y, I2131SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
LRRK2_1867_2161_L1976Y_I2049Q_F2083A
Plasmid#40451DepositorInsertLRRK2 (LRRK2 Human)
UseBaculovirusTagsGST-Tev and HisTevExpressionMutationaa1867_2161; L1976Y, I2049Q, F2083APromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET22b-EC12WTC9AAvi
Plasmid#47925PurposeExpresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.DepositorInsertE-Cadherin EC12
UseTags6xHis, Avi Tag, and TEV CleavageExpressionBacterialMutationchanged Cysteine 9 to AlaninePromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EC12WTC9A
Plasmid#47924PurposeExpresses E-cadherin EC12 WT with C9A mutation in E.Coli.DepositorInsertE-Cadherin EC12
UseTags6xHis and TEV CleavageExpressionBacterialMutationchanged Cysteine 9 to AlaninePromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
5WCI (MYST1)
Plasmid#101269PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMYST1 (KAT8 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Cys-60nmSAH-SPYcatcher
Plasmid#214840PurposeThis plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-60nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cys-30nmSAH-SPYcatcher
Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pNIC28-Bsa4
Plasmid#26103PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis - TEVExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NHSTIIT-AVITAG-GG
Plasmid#215802PurposeCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAGDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTF101.1
Plasmid#134770PurposeA binary plasmid containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorArticleTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -