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Plasmid#176135PurposeHeterodimerization with FRB (rapamycin), Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseEntry vectorTagsExpressionMutationSee Depositor Comments BelowPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso CdelET
Plasmid#137723PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)DepositorInsertBRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDeleted amino acids 600-682PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseTags3x HAExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4-2 WW2
Plasmid#111883PurposeMode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: NEDD4-2 WW2 fused to C-term…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-P/PM,4A5C6G7G-GFP
Plasmid#80914PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationPromoterP4A5C6G7G and PM,4A5C6G7GAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mcherry-WIPI2d-cs-TEV-STREP
Plasmid#178912PurposeMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal StrepDepositorInsertWIPI2d
UseTagsC term strep and N term mcherryExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/UAA-mEGFP-IRES-mCherry
Plasmid#49225PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
G30, Cdc20-mCherry-AID tagging
Plasmid#130270PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombinationDepositorInsertCdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd
Plasmid#118278PurposeExpresses bPAC(WT)-mCherry under a minimal CamKII PromoterDepositorInsertbPAC(WT)
UseAAVTagsmCherry and mycExpressionMutationPromoterCamKIIAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-F14F15S-BFP2-(mCherry)'
Plasmid#115235PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after itDepositorInsertmTagBFP2 and mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti
Plasmid#138318PurposeLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFPDepositorInsertSauCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-NLS-BirA-2A-mCherry_Ras
Plasmid#80065PurposeZic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterZic2a enhancer driving c-fos promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti pTERT sgRNA2(MS2) mCherry
Plasmid#171055PurposeTargeting of Vp64-dCas9 activation system to hTERT promoterDepositorInsertpTERT sgRNA2(MS2) mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_4'_+nls-mCherry-Flag-_+1_BK+
Plasmid#49313PurposeGolden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codonDepositorInsertnls-mCherry-Flag
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNTI757 P(PGK1)-citrine P(HIS4)-mCherry
Plasmid#164917PurposeDivergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizerDepositorInsertP(PGK1)-citrine and P(HIS4)-mCherry
UseTagsExpressionYeastMutationPromoterPGK1 (yeast) and HIS4 (yeast)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
KY901: pMVP (L3-L2) P2A-mCherry + polyA
Plasmid#121766PurposepMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.DepositorInsertP2A-mCherry + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC1-mCherry-H2B-Venus
Plasmid#129559Purposenuclear markerDepositorInsertmcherry and Histone 2B fused to venus
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LA001: pMVP (L3-L2) P2A-mCherry + WPRE
Plasmid#121774PurposepMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-mCherry + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54(L53P,L67P)
Plasmid#172458PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutantDepositorInsertTPD54 (TPD52L2 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationchanged Leucines 53 and 67 to proline (breaks coi…PromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only