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pBRY-nuclear mCherry-IRES-PURO

Plasmid

#52409

Purpose

Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

nuclear mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123A)-mCherry

Plasmid

#35506

Purpose

AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV9

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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61426-hU6-shmFmr1-EF1a-mCherry

Plasmid

#157859

Purpose

Expresses a shRNA targeting mouse Fmr1 gene

Depositor

Xinyu Zhao

Article

Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

Insert

Fmr1 (Fmr1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-shSCR

Plasmid

#29780

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

shSCR

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pDisplay-UDP1.0-IRES-mCherry-CAAX

Plasmid

#220850

Purpose

CMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherry

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-OPTN(F178A/D474N)

Plasmid

#119684

Purpose

For mammalian expression of LIR/UBD double mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

F178A/D474N

Promoter

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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p1xNLS-FRB-mCherry-hsp86-BSD

Plasmid

#85795

Purpose

Expression of 1xNLS mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

1xNLS Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Expression

Mutation

Promoter

Pfhsp86

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

Plasmid

#181851

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Lysine 128 in the DnaJB1-PKAcat fusion changed to…

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-GLI1-P2A-mCherry

Plasmid

#203858

Purpose

Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

GLI1 (GLI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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tol2-4xnr UAS-PA Rac1-mCherry

Plasmid

#41878

Depositor

Anna Huttenlocher

Insert

photoactivatable Rac1 (RAC1 Human)

Use

Zebrafish expression

Tags

6x His and mCherry

Expression

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-FKBP-GEF(ITSN1)

Plasmid

#222631

Purpose

Cdc42 activation using rapamycin

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT-PonA-BI-Gl-GFP-WT-mCherry-WT (5FP-WW)

Plasmid

#189794

Purpose

Express GFP/mCherry linked wild-type beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Human, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ponasterone A inducible

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS3-Syn21-V5-mCherry-P10

Plasmid

#104617

Purpose

Expresses V5 tagged mCherry upon binding of TALE3 to VAS3

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

Syn21-V5-mCherry-P10-MhcSA

Use

Tags

V5

Expression

Insect

Mutation

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-mCherry

Plasmid

#137134

Purpose

Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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P-rTetO-mCherry-T2A-RPL22-1xV5

Plasmid

#170325

Purpose

Tetracycline-inducible expression of V5 and mCherry tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMK447 (BSD-P2A-BromoTag-mCherry)

Plasmid

#214377

Purpose

BromoTag-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-KLF4-P2A-mCherry

Plasmid

#203855

Purpose

Tet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

KLF4 (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#2-mCherry_PGK-H2B-CFP

Plasmid

#164102

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO2-TVA-mCherry

Plasmid

#149297

Purpose

Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

TVA:mCherry

Use

AAV

Tags

Expression

Mutation

Start codon removed.

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-mCherry-attP(C31)-*BleoR

Plasmid

#183752

Purpose

phiC31 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

FRT-mCherry-attP-BleoR-F3

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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