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pDEST27-GST-ATXN1 FL(84Q)-S776A

Plasmid

#21758

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 84 Q(Glutamines) and…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pDEST27-GST-ATXN1 FL(30Q)-S776A

Plasmid

#21756

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pKM-U6-reRNA-hsEMX1

Plasmid

#205637

Purpose

reRNA guide targeting Homo sapiens EMX1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsEMX1 (EMX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSFFV-IGKleader-hIgG1_FC-Myc-PDGFRb-IRES2-EGFP (MCS reporter)

Plasmid

#222998

Purpose

MCS reporter constitutively expressing EGFP and conditionally expressing a synthetic cell surface protein upon prime editing. For lentivirus packaging.

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

IGKleader-hIgG1_FC-Myc-PDGFRb-IRES2-EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

Plasmid

#189636

Purpose

Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_4-1317_S

Plasmid

#147537

Purpose

Mammalian Expression of HsNot1iso1_4-1317

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_4-1317 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_4-1580_S

Plasmid

#147538

Purpose

Mammalian Expression of HsNot1iso1_4-1580

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_4-1580 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_4-1317_S

Plasmid

#147531

Purpose

Mammalian Expression of HsNot1iso1_4-1317

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_4-1317 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-del1147-1717-dsRNAres_R

Plasmid

#147434

Purpose

Insect Expression of DmNot1-del1147-1717-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1-del1147-1717-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

8 silent mutations and two non silent N605S, K759…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-4xmut-dsRNAres_R

Plasmid

#147425

Purpose

Insect Expression of DmCCR4-4xmut-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-4xmut-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_1-166-L42EI44E-dsRNAres_R

Plasmid

#147426

Purpose

Insect Expression of DmCCR4_1-166-L42EI44E-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_1-166-L42EI44E-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-L42EI44E-dsRNAres_R

Plasmid

#147429

Purpose

Insect Expression of DmCCR4-L42EI44E-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-L42EI44E-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1-1709-dsRNAres_R

Plasmid

#147430

Purpose

Insect Expression of DmNot1_1-1709-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1709-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-4x mut_R

Plasmid

#147371

Purpose

Mammalian Expression of HsNot1iso1-4xmut

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-4xmut (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-K1208AH1212AK1218A_R

Plasmid

#147372

Purpose

Mammalian Expression of HsNot1iso1-K1208AH1212AK1218A

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-K1208AH1212AK1218A (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot1iso1-K1208EH1212TK1218E_R

Plasmid

#147373

Purpose

Mammalian Expression of HsNot1iso1-K1208EH1212TK1218E

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-K1208EH1212TK1218E (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-P1209YP1257Y_R

Plasmid

#147374

Purpose

Mammalian Expression of HsNot1iso1-P1209YP1257Y

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-P1209YP1257Y (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-P1257Y_R

Plasmid

#147375

Purpose

Mammalian Expression of HsNot1iso1-P1257Y

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-P1257Y (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-K1277A-dsRNAres_R

Plasmid

#147359

Purpose

Insect Expression of DmNot1-K1277A-dsRNAres

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

DmNot1-K1277A-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1-Q1271A-dsRNAres_R

Plasmid

#147360

Purpose

Insect Expression of DmNot1-Q1271A-dsRNAres

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

DmNot1-Q1271A-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge