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Plasmid#175518PurposeFor mammalian expression of adenovirus VAI RNADepositorInsertAd VAI RNA
UseAdenoviralTagsExpressionMutationPromoterlac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSin Helper
Plasmid#127694PurposePlasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Glycoproteins
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSin Capsid
Plasmid#127693PurposePlasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Capsid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXs-Mllt3
Plasmid#131604Purposeexpression of mouse MIIt3 in mammalian cellsDepositorInsertMllt3 (Mllt3 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
FSW-ALDH2-E504K-T2A-EGFP
Plasmid#197037PurposeExpress ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFPDepositorInsertALDH2-E504K
UseLentiviralTagsExpressionMammalianMutationE504KPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-HBB-nLuc
Plasmid#175432PurposeUsed to generate IVT RNA for translation extractsDepositorInsertHBB-nLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hORAI1sg
Plasmid#174326PurposegRNA targeting hORAI1 - encodes puromycin resistanceDepositorInserthuman Orai1 (ORAI1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HS-OS-Plk4-mKate2
Plasmid#105944Purposeheat shock inducible transgenesis, non phoshorylable version of Plk4DepositorInsertPlk4 (Plk4 Mouse)
UseTagsmKate2ExpressionBacterialMutation1002 to 3704 of Plk4/ deletion of S282-S305.PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562A
Plasmid#191976PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T562A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T562D
Plasmid#191977PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T562D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT562DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
HS-aPKC-CAAX-GFP
Plasmid#105946Purposeheat shock inducible transgenesisDepositorInsertaPKC (Prkcz Rat)
UseTagsCAAX and GFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-Venus-173-N
Plasmid#87616PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-Venus173-N
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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cmamxA1-GFP
Plasmid#107195PurposeExpresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deletedDepositorInsertcmannexin A1
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationexchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T564A
Plasmid#191978PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T564A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT564APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T564D
Plasmid#191979PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T564D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT564DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-DMVF-181-N
Plasmid#87610PurposeFRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-DMVF181-N
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
UseTagsExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Venus-Actb
Plasmid#169788PurposeAn actin FRET sensor. Must be expressed with ECFP-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SMAD2_HAe2_Bsd
Plasmid#167166PurposeBlasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.DepositorInsertRepair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
UseRepair template for gene editing in human cellsTagsblasticidinExpressionMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only