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pBTK403

Plasmid

#110599

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK401

Plasmid

#110597

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAT9658-sgRNA-mCherry

Plasmid

#162987

Purpose

sgRNA cloning plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

exchangeable cassette

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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PB-BE3-2NLS

Plasmid

#119031

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pKV014

Plasmid

#64765

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, 3xHA to 3xT7-mRFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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PB-BE3-3NLS

Plasmid

#119032

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEY136

Plasmid

#223483

Purpose

tag-168 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tag-168

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEY106

Plasmid

#223453

Purpose

nsf-1 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

nsf-1 (nsf-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY143

Plasmid

#223490

Purpose

unc-31 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-31 (unc-31 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pWR76

Plasmid

#202776

Purpose

episomal fluorescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBC127

Plasmid

#202254

Purpose

Level 2 for strain-specific barcode DNA tag with bc154 through Tn7 bacterial insertion, Kanamycin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc154

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC128

Plasmid

#202255

Purpose

Level 2 for strain-specific barcode DNA tag with bc155 through Tn7 bacterial insertion, Kanamycin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc155

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC129

Plasmid

#202256

Purpose

Level 2 for strain-specific barcode DNA tag with bc156 through Tn7 bacterial insertion, Kanamycin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc156

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC069

Plasmid

#202233

Purpose

Level 2 for strain-specific barcode DNA tag with bc096 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc096

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC070

Plasmid

#202234

Purpose

Level 2 for strain-specific barcode DNA tag with bc097 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc097

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC071

Plasmid

#202235

Purpose

Level 2 for strain-specific barcode DNA tag with bc098 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc098

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC072

Plasmid

#202236

Purpose

Level 2 for strain-specific barcode DNA tag with bc099 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc099

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBC073

Plasmid

#202237

Purpose

Level 2 for strain-specific barcode DNA tag with bc100 through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc100

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBCC088

Plasmid

#202215

Purpose

Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and tagRFP fluorescent marker

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

pBCC25, pBCC33, pBCC58, pBCC27, pBCC26, pBCC28, pICH41822 (G5)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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