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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pmx-MiGT

Plasmid

#172397

Purpose

Retroviral expression vector for murine direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

Insert

Mef2c-i, Gata4, Tbx5 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Neuropeptide Y [L115/13R-2b]

Plasmid

#206639

Purpose

Mammalian Expression Plasmid of anti-Neuropeptide Y (Human). Derived from hybridoma L115/13-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Neuropeptide Y (Homo sapiens) recombinant Mouse monoclonal antibody (NPY Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-3xFLAG::Fnip1-pgk-hph

Plasmid

#48758

Purpose

PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

3xFLAG::Fnip1 (Fnip1 Mouse)

Use

Piggybac

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only
Anti-Slo1/BKAlpha maxi-K+ channel (Mouse) [L6/23R]

Plasmid

#128634

Purpose

Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/23.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Slo1/BKAlpha maxi-K+ channel (Mouse) recombinant mouse monoclonal antibody (Kcnma1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pINTO-NSA::mEzh2

Plasmid

#61725

Purpose

Expression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)

Depositor

Roberto Bonasio

Article

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Insert

Enhancer of zeste homolog 2 (Ezh2 Mouse)

Use

Tags

mSA (monomeric streptavidin)

Expression

Mammalian

Mutation

Promoter

T-REx (CMV + 2xTetO2)

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP D161N

Plasmid

#41383

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D161N)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
mCherry-Alpha1-Catenin-N-18

Plasmid

#54977

Purpose

Localization: Tight Junctions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Alpha1-Catenin

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Hes5

Plasmid

#61536

Purpose

Expresses Hes5 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes5 (Hes5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
Parvalbumin scFv [L114/3]

Plasmid

#182072

Purpose

Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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9E10.mCHOP(S78&81A).pCDNA1/Amp

Plasmid

#21902

Depositor

David Ron

Insert

CHOP (Ddit3 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

S78A S81A

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1MKK7B2

Plasmid

#19731

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

Jnk1a1MKK7b2 (Map2k7, MAPK8 Human, Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
p3XFlag-Mettl4

Plasmid

#86665

Purpose

Expresses murine Mettl4 containing N-terminal 3XFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Mettl4 (Mettl4 Mouse)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-shEsr2

Plasmid

#120722

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

Depositor

Amy Lasek

Article

Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

Insert

Esr2 (Esr2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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TRE.IkBSR-Flag Pgk.Cre

Plasmid

#22504

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

IkBSR

Use

Cre/Lox and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-E14-ChR2GFP2x

Plasmid

#153435

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E14 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GluN3A/NR3A glutamate receptor scFv [N416/57]

Plasmid

#206773

Purpose

Mammalian Expression of GluN3A/NR3A glutamate receptor scFV. Derived from hybridoma N416/57 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN3A/NR3A glutamate receptor (Mus musculus) recombinant scFV (Grin3a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Neuroligin-2 scFv [L107/90]

Plasmid

#206763

Purpose

Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/36]

Plasmid

#190563

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFV. Derived from hybridoma N59/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN2B/NR2B glutamate receptor (Rattus norvegicus) recombinant scFV (Grin2b Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBSWHhc-p53

Plasmid

#133903

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (TP53 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6-2 SNAP-Syk tSH2

Plasmid

#113028

Purpose

for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

SNAP-Syk tSH2

Use

Tags

SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK4alpha KD

Plasmid

#12188

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K1361M

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
-2.5Kb MMP10

Plasmid

#53973

Purpose

Luciferase reporter for mouse MMP10 2.5kb promoter

Depositor

Michael Chin

Article

Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

Insert

mouse MMP10 2.5Kb promoter (Mmp10 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PCR from C57BL6/J

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta2-3xFiBsd

Plasmid

#140964

Purpose

full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse)

Use

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-KIF13A tail 361-1749

Plasmid

#64288

Purpose

KIF13A aa 361-1749 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

KIF13A (Kif13a Mouse)

Use

Tags

3myc and FRB

Expression

Mammalian

Mutation

aa361-1749

Promoter

chicken Beta Actin

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Nipbl_Neo_FKBPF36V_mScarlet

Plasmid

#163883

Purpose

Acute Depletion NIPBL-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Nipbl (Nipbl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Mouse Alpha2 delta-3 HA pMT2

Plasmid

#58728

Purpose

expression of HA tagged mouse Alpha2 delta-3 in mamalian cells

Depositor

Annette Dolphin

Article

Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

Insert

alpha2 delta-3 (Cacna2d3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

Plasmid

#181814

Purpose

Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc5b D1 (Muc5b Mouse)

Use

Tags

TEV cleavage site followed by 6xHistidine tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PU.1-ets-IRES-mCherry

Plasmid

#80141

Purpose

retroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

PU.1 (Spi1 Mouse)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Mutation

ets = PU.1 DNA binding domain (amino acids 159-26…

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR macroH2A1.2-C

Plasmid

#30512

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6. ():.

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Gateway - donor vector

Tags

Expression

Mutation

NO STOP

Promoter

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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