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Plasmid#129744PurposeExpresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cellsDepositorInsertORF31 (ORF31 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags2xStrep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC119
Plasmid#63916PurposeRecipient overexpressing vector to construct FLAG tagged fusionsDepositorTypeEmpty backboneUseTagsFLAGExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192396PurposeTo act as a 2-input state of the AND gate.DepositorInsertAct2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192391PurposeTo act as a 2-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST(frt)(ATG_V5-APEX2_noSTOP)
Plasmid#168207PurposeDestination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selectionDepositorTypeEmpty backboneUseUnspecified; Pdest gatewayTagsV5 APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ORF30-2xStrep pCDNA4
Plasmid#129743PurposeExpresses KSHV ORF30 with Cterm 2xStrep tag in mammalian cellsDepositorInsertORF30 (ORF30 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags2xStrep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xStrep-ORF34 pCDNA4
Plasmid#120376PurposeExpresses KSHV ORF34 with Nterm 2xStrep tag in mammalian cellsDepositorInsertORF34 (ORF34 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags2xStrep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18-3xFLAG pCDNA4
Plasmid#120372PurposeExpresses KSHV ORF18 with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7woH
Plasmid#65020PurposeExpresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ
Plasmid#192427PurposeTo act as the on state for testing of the alt Vika recombinase.DepositorInsertAct2::vox-OCS-vox::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192432PurposeTo act as a 2-input state of the NOR gate.DepositorInsertAct2::FRT-B3RT-Rluc-B3RT-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA4CHmneo
Plasmid#69607PurposeExpresses a neomycin tagged codon optimized L1PA4DepositorInsertL1PA4
UseTagsmneo retrotransposition cassetteExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
double_sgRNA targeting e3 and e7
Plasmid#190689PurposesgRNAs targeting enhancer 3 and 7 of MYC separatelyDepositorInsertsgRNAs targeting enhancer 3 and 7 of MYC separately
UseCRISPR, Lentiviral, and Synthetic BiologyTagsBFP and PuromycinExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK7 wt
Plasmid#80885Purposemammalian expression of ALK7 WTDepositorInsertALK7 (Acvr1c Rat)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB-HA no-pA
Plasmid#82594Purpose3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
HcKan_T
Plasmid#65316Purposereceiving vector of terminators for the construction of standard terminator parts in YeastFab workDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
HcKan_O
Plasmid#65315Purposereceiving vector of ORFs for the construction of standard ORF parts in YeastFab workDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
HcKan_P
Plasmid#65314Purposereceiving vector of promoters for the construction of standard promoter parts in YeastFab workDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre
Plasmid#192421PurposeTo act as the 2-input state of the OR gate.DepositorInsertAct2::FRT-loxP-OCS-FRT-loxP::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only