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Showing: 2881 - 2900 of 72239 results

  3. pODN-H2BC11-mR2

    Plasmid
    #183866
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mRuby2
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183866-plasmid-map-sequence-id-368215

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  4. pODN-mR2-MYH10

    Plasmid
    #183869
    Purpose
    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183869-plasmid-map-sequence-id-362448

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  5. pODN-H2BC11-TagBFP

    Plasmid
    #183867
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mTagBFP
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183867-plasmid-map-sequence-id-362447

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  6. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

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  7. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid
    #82473
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Article
    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d
    Insert
    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
    Use
    Tags
    TEV-Biotin and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82473-plasmid-map-sequence-id-163076

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  8. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

    Plasmid
    #50866
    Purpose
    Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50866-plasmid-map-sequence-id-76339

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  9. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid
    #50875
    Purpose
    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I674C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50875-plasmid-map-sequence-id-76348

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  10. pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)

    Plasmid
    #50870
    Purpose
    Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    N680C A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50870-plasmid-map-sequence-id-76343

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  11. pcDNA3.1 Flag YFP hNKCC1 L737C (NT523)

    Plasmid
    #50891
    Purpose
    Expresses human NKCC1 L737C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L737C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50891-plasmid-map-sequence-id-76364

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  12. pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)

    Plasmid
    #50867
    Purpose
    Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50867-plasmid-map-sequence-id-76340

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  13. pcDNA3.1 Flag YFP hNKCC1 N672C (NT474)

    Plasmid
    #50873
    Purpose
    Expresses human NKCC1 N672C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    N672C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50873-plasmid-map-sequence-id-76346

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  14. pcDNA3.1 Flag YFP hNKCC1 P676C (NT443)

    Plasmid
    #50877
    Purpose
    Expresses human NKCC1 P676C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50877-plasmid-map-sequence-id-76350

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  15. pcDNA3.1 Flag YFP hNKCC1 N731C (NT505)

    Plasmid
    #50885
    Purpose
    Expresses human NKCC1 N731C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    N731C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50885-plasmid-map-sequence-id-76358

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  16. pcDNA3.1 Flag YFP hNKCC1 V673C (NT437)

    Plasmid
    #50874
    Purpose
    Expresses human NKCC1 V673C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    V673C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50874-plasmid-map-sequence-id-76347

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  17. pcDNA3.1 Flag YFP hNKCC1 I677C (NT467)

    Plasmid
    #50878
    Purpose
    Expresses human NKCC1 I677C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I677C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50878-plasmid-map-sequence-id-76351

    Enlarge

  18. pcDNA3.1 Flag YFP hNKCC1 I678C (NT445)

    Plasmid
    #50879
    Purpose
    Expresses human NKCC1 I678C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I678C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50879-plasmid-map-sequence-id-76352

    Enlarge

  19. pcDNA3.1 Flag YFP hNKCC1 L736C (NT522)

    Plasmid
    #50890
    Purpose
    Expresses human NKCC1 L736C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L736C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50890-plasmid-map-sequence-id-76363

    Enlarge

  20. pcDNA3.1 Flag YFP hNKCC1 A675C (NT441)

    Plasmid
    #50876
    Purpose
    Expresses human NKCC1 A675C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A675C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50876-plasmid-map-sequence-id-76349

    Enlarge

Showing: 2881 - 2900 of 72239 results