Addgene

Showing: 2881 - 2900 of 4607 results

Results per page:

pCAG-T3-st1CAS9-pA

Plasmid

#131542

Purpose

Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

Insert

Codon optimized st1Cas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC6A2

Plasmid

#131891

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Sijben et al Sci Rep. 2021 Jun 10;11(1):12290. doi: 1

Insert

SLC6A2 (SLC6A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVAX_CBI_AcrIIA2

Plasmid

#136661

Purpose

Expresses human codon-optimized AcrIIA2 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA2

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA4

Plasmid

#136662

Purpose

Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA4

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA5_D1126

Plasmid

#136663

Purpose

Expresses human codon-optimized AcrIIA5 D1126 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA5_D1126

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA5_D4276

Plasmid

#136664

Purpose

Expresses human codon-optimized AcrIIA5 D4276 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA5_D4276

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA6_D1024

Plasmid

#136666

Purpose

Expresses human codon-optimized AcrIIA6 D1024 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_D1024

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA6_D1811

Plasmid

#136667

Purpose

Expresses human codon-optimized AcrIIA6 D1811 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_D1811

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pVAX_CBI_AcrIIA6_DT1

Plasmid

#136668

Purpose

Expresses human codon-optimized AcrIIA6 DT1 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA6_DT1

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
prk5-MFSD12-TEV-3xFLAG-3xHA

Plasmid

#149504

Purpose

Transient expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Transient expression

Tags

TEV-3XHA-3XFLAG

Expression

Mammalian

Mutation

Codon Optimized Sequence

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-HIV-1 Group O-vpr

Plasmid

#115836

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group O Vpr (L20571)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-HIV-1 Group P-vpr

Plasmid

#115837

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group P Vpr (HQ179987)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

Plasmid

#117720

Purpose

Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

mAID-nanobody
IBB-mCherry

Use

Tags

importin-beta bining domain (IBB) and triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV and None, IRES-driven

Availability

Academic Institutions and Nonprofits only

Enlarge
evoCas9

Plasmid

#138558

Purpose

Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

evoCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

M495V, Y515N, K526E, R661Q

Promoter

EFS

Availability

Academic Institutions and Nonprofits only

Enlarge
VQR variant

Plasmid

#138560

Purpose

Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VQR

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

EFS

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1-PRD(703-800)-Strep

Plasmid

#141344

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1-His6-TEV-ALIX(703-800)-Strep

Use

Tags

GB1-His6-TEV and Strep-tag (WSHPQFEK)

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-PfROM4 (G470A/G471N)

Plasmid

#154859

Purpose

Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfROM4 (PF3D7_0506900 Synthetic)

Use

Tags

triple HA

Expression

Mammalian

Mutation

Glycine 470 changed to Alanine and Glycine 471 ch…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-PfROM1 (A144G/N145G)

Plasmid

#154873

Purpose

Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfROM1 (PF3D7_0506900 Synthetic)

Use

Tags

triple HA

Expression

Mammalian

Mutation

Alanine 144 changed to Glycine and Asparagine 145…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1 ISCU D71A

Plasmid

#169880

Purpose

Expresses ISCU iron-sulfur cluster trapping mutant (dominant negative)

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

ISCU

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Codon Optimized, D71A iron-sulfur cluster trappin…

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

Enlarge
pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Article

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Use

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge