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Plasmid#169460PurposeEncodes SARS-CoV-2 B.1.1.298 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 B.1.1.298 Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-BioID2-HA
Plasmid#130980PurposeExpression of HA-tagged KIF1C-BioID2DepositorInsertKIF1C-BioID2-HA (KIF1C Human)
UseTagsBioID2-HAExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-WT
Plasmid#91986PurposeExpresses FLAG-HA-AGO2 (WT)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Dnmt3b6-Poly(A)
Plasmid#65532PurposePlasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locusDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationisoform 6PromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 P.2/B.1.1.248
Plasmid#173477PurposeEncodes SARS-CoV-2 P.2/B.1.1.248 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 Spike Truncated to remove 18 amino acids
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)
Plasmid#156164PurposeExpresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequenceDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationTHBS1 with addition of ER-retention sequence KDELPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pT7-7 asyn S87E
Plasmid#36059DepositorInsertalpha-synuclein S87E (SNCA Human)
UseTagsExpressionBacterialMutationS87EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-dKARD
Plasmid#114053Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-dKARD (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance): AGATACTAGCT…PromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST15.1-hRPN13 (285-386)
Plasmid#61936PurposeExpression of hRPN13 (285-386) in E.coliDepositorInserthRPN13 (285-386) (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationConstruct starts at residue 285, stop codon intro…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(92Q)-S776D
Plasmid#21757DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains a stretch of 92 Q(Glutamines) and…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A
Plasmid#36058DepositorInsertalpha-synuclein S87A (SNCA Human)
UseTagsExpressionBacterialMutationS87APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1362 9xS/A
Plasmid#24373DepositorInsertCLASP2 (340-1362) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(30Q)-S776D
Plasmid#21755DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains a stretch of 32 amino acids (30 Q…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)
Plasmid#113041PurposeAAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)DepositorInsertgRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only