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Plasmid#74837PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only
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pHIV-H2B-eGFP
Plasmid#91776PurposeExpresses H2B protein tagged with eGFPDepositorInsertHuman Histone H2B / eGFP (H2BC11 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB553
Plasmid#74883PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-USP47
Plasmid#64326PurposeMammalian expression and purification of USP47.DepositorInsertUSP47 (USP47 Human)
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCX-NTOM-SpiCee
Plasmid#140902Purposemammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertNTOM-SpiCee
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEY11
Plasmid#191017PurposeFluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)DepositorInsertUltrapanneuronal
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK402
Plasmid#110598PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB453
Plasmid#74835PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB435
Plasmid#68700PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-AKARev
Plasmid#118478PurposeddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.DepositorInsertRAB-AKARev
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-RAB-EKARev
Plasmid#118477PurposeddRFP-based single-color ERK biosensor targeted to the plasma membrane.DepositorInsertLyn-RAB-EKARev
UseTagsN-terminal targeting sequence from Lyn-kinaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
trPAGmEos3.2
Plasmid#203752PurposeEncodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrPAG (PAG1 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-NES
Plasmid#140901Purposemammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-NES
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB426
Plasmid#68691PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab11
Plasmid#174460PurposeExpress iRFP with recycling endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with recycling endosome-targeting sequence
UseTagssmall GTPase (Rab11)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCX-Lyn-SpiCee
Plasmid#140837Purposemammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertLyn-SpiCee
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-Kras
Plasmid#140838Purposemammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-Kras
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS:bloSwitch
Plasmid#108355PurposeUAS driven green to red switch reporter for B3 recombinase expressionDepositorInsertUAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
UseZebrafish expressionTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only