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124_pAAV-ProC3-CatCh-GFP-WPRE

Plasmid

#125939

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC3

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-V5-SHLD3

Plasmid

#114124

Purpose

Expresses N-terminally tagged V5-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 23, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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151_pAAV-ProC29-CatCh-GFP-WPRE

Plasmid

#125964

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC29

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-ProA5-GCaMP6s

Plasmid

#126003

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

ProA5

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-i53-DM

Plasmid

#92171

Purpose

recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

i53-DM

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

UbvG08 with I44A, P69L, and L70V mutations and no…

Promoter

CMV

Available since

July 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
161_pAAV-ProD5-CatCh-GFP-WPRE

Plasmid

#125981

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD5

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
III_pAAV-ProB4-CatCh-GFP-WPRE

Plasmid

#125924

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProB4

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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17_pAAV-ProB1-CatCh-GFP-WPRE

Plasmid

#125921

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProB1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-IDR1-mCherry-Cry2

Plasmid

#177125

Purpose

Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (IDR1 domain) (YTHDC1 Human)

Use

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

IDR1 domain only

Promoter

SFFV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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107_pAAV-ProC17-CatCh-GFP-WPRE

Plasmid

#125952

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC17

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

Plasmid

#105801

Purpose

Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: FLAG-3C; ORF2: mClover3-TEV

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 2)

Plasmid

#99562

Purpose

mammalian expression of TMEM230-GFP (isoform 2)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
113_pAAV-ProC21-CatCh-GFP-WPRE

Plasmid

#125956

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC21

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
119_pAAV-ProC25-CatCh-GFP-WPRE

Plasmid

#125960

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC25

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG

Plasmid

#74940

Purpose

Ubiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

Ubiquitin

Use

Tags

Flag

Expression

Mammalian

Mutation

UbvG08 with I44A, P69L, and L70V mutations and no…

Promoter

CMV

Available since

May 13, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
62_pAAV-ProA19-CatCh-GFP-WPRE

Plasmid

#125902

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA19

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
60_pAAV-ProA17-CatCh-GFP-WPRE

Plasmid

#125900

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA17

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
3_pAAV-ProA8-CatCh-GFP-WPRE

Plasmid

#125892

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA8

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

Plasmid

#105805

Purpose

Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: 3C-Cerulean; ORF2: TEV-Venus

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pVZ_PcoaT_SQE1_LUP1

Plasmid

#103857

Purpose

Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803

Depositor

Ilka Axmann

Article

Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

Insert

lupeol synthase 1 (LUP1 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

inserted genes are codon optimized. 2nd amino aci…

Promoter

PcoaT (from Synechocystis sp. PCC 6803)

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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