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pCDEF3‐hTIM3 (no tag)
Plasmid#49212Purposeexpresses human Tim3DepositorInsertTim3 (HAVCR2 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Ace-mNeon2-Kv2.1PR
Plasmid#195528PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-pAceR-Kv2.1PR
Plasmid#195525PurposeRed fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterSynapsinAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-VARNAM2-Kv2.1PR
Plasmid#195533PurposeCre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxTagsExpressionMammalianMutationVARNAM SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
hGATA-1 K137R
Plasmid#118353PurposeThis plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.DepositorInserthGATA-1 (GATA1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK137RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Ace-mNeon2-Kv2.1PR
Plasmid#195522PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterSynapsinAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-pAceR-Kv2.1PR
Plasmid#195531PurposeRed fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-CHAMP1
Plasmid#159892PurposeExpresses CHAMP1 (CAMP) in mammalian cellsDepositorInsertCHAMP1 (CHAMP1 Human)
UseTagspEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
POLR2A-N CRISPR pX330
Plasmid#124495PurposeA CRISPR plasmid for targeting the N-terminus coding region of human POLR2ADepositorInsertPOLR2A targeting CRISPR
UseCRISPRTagsExpressionMutationPromoterU6 promoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K298R
Plasmid#118362PurposeThis plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK298RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL838
Plasmid#70680PurposeThis vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330 NL-FKBP-Cas9
Plasmid#162723PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pITR-AAV-Rep2Cap9-GFP
Plasmid#199744PurposeExpresses AAV2 Rep and AAV9 Cap, with GFP replacing a region of Cap, within AAV2 ITRs.DepositorInsertAAV9 Cap w/ GFP insert
UseAAVTagsExpressionMammalianMutationReplaced amino acids 356-736 in Cap with GFPPromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA-hNEUROG2-iRPT
Plasmid#140764PurposeExpresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330 N-FKBP-Cas9
Plasmid#162727PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR-U6gRNA-PGKpuro2ABFP
Plasmid#136405PurposeDonor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationBbsI restriction site at position 437 was removed…PromoterU6, PDKAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.Flag.HERC2-HECT-C4762A.6xHis
Plasmid#39228DepositorInsertHERC2 HECT Domain (HERC2 Human)
UseTags6xHis and FlagExpressionMammalianMutationC4762APromoterT7Available sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
MO70-hJunctate
Plasmid#79596PurposeTransient and retroviral expressionDepositorInsertjunctate (ASPH Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only