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pDONR223_IKZF5_WT

Plasmid

#81855

Purpose

Gateway Donor vector containing IKZF5 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKZF5 (IKZF5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

M204V; p.415_418delQHNQ; p.419_420-insITNID

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAM116A_p.R427H

Plasmid

#81626

Purpose

Gateway Donor vector containing FAM116A , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FAM116A (DENND6A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R427H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXO15_p.S366G

Plasmid

#81409

Purpose

Gateway Donor vector containing FBXO15 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXO15 (FBXO15 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S366G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IKZF5_p.E135K

Plasmid

#81384

Purpose

Gateway Donor vector containing IKZF5 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKZF5 (IKZF5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E135K; M204V; p.415_418delQHNQ; p.419_420-insITNID

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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HK3 gRNA (BRDN0001146645)

Plasmid

#75779

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK3 (HK3, HOOK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HK3 gRNA (BRDN0001148398)

Plasmid

#75780

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK3 (HK3, HOOK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HK3 gRNA (BRDN0001149028)

Plasmid

#75781

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK3 (HK3, HOOK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HK3 gRNA (BRDN0001149156)

Plasmid

#75782

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK3 (HK3, HOOK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a QTY

Plasmid

#162442

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ146a QTY

Plasmid

#162443

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a-sfGFP

Plasmid

#162446

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

truncation from aa52 to aa257

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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PB-tetO-hNIL

Plasmid

#197089

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLENTI_hACE2_HygR

Plasmid

#155296

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

Depositor

Raffaele De Francesco

Article

Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Expression

Mutation

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-N EBNA3A

Plasmid

#37956

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNA3A (EBNA-3A H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

PKG

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-BSG-Fc(DAPA)-AviTag-6xHis

Plasmid

#156742

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

BSG (BSG Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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G protein alpha-o-YFP

Plasmid

#55776

Purpose

This G protein alpha-o construct contains internal insertions of YFP and the EE epitope

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)

Use

Tags

EE epitope was introduced internally with D167E a…

Expression

Mammalian

Mutation

A BglII site was removed from the 5' untrans…

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-GluK5/Grik5/KA2 kainate receptor [N279B/27R]

Plasmid

#149451

Purpose

Mammalian Expression Plasmid of anti-GluK5/Grik5/KA2 kainate receptor (Rat). Derived from hybridoma N279B/27.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GluK5/Grik5/KA2 kainate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grik5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-BBS3/Arl6 [N302/10R]

Plasmid

#188211

Purpose

Mammalian Expression Plasmid of anti-BBS3/Arl6 (Human). Derived from hybridoma N302/10

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-BBS3/Arl6 (Homo sapiens) recombinant mouse monoclonal antibody (ARL6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FUS_FLI1

Plasmid

#205822

Purpose

Express mEGFP-tagged fusion protein, FUS_FLI1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

FUS_FLI1 (FUS, FLI1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha2 [N399/19R]

Plasmid

#177544

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha2 (Rat). Derived from hybridoma N399/19.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Alpha2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabra2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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