-
Plasmid#213469PurposeGateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cda-32aa linker-z…TagsNoExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-SpRY
Plasmid#161520PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoterDepositorInsertzSpRYCas9
UseCRISPR; Gateway compatible zsprycas9 entry cloneTags3X FLAG, NLS and NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262-ABE8e
Plasmid#161523PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editingDepositorInsertABE8e-zSpCas9(D10A)
UseCRISPR; Gateway compatible abe8e-zspcas9(d10a) en…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM190
Plasmid#69614Purposeinducible gene expression in StreptomycesDepositorInsertsaphII
tsr
rep
sso
TipA promoter
dso
UseStreptomyces- e. coli shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266E
Plasmid#161521PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editingDepositorInsertzSpRYCas9(D10A)-PmCDA1-UGI
UseCRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…Tags3X FLAG, NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262B-ABE8e
Plasmid#161524PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editingDepositorInsertTadA8e-zSpRYCas9(D10A)
UseCRISPR; Gateway compatible tada8e-zsprycas9(d10a)…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)
Plasmid#191650PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265F2
Plasmid#164722PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)NG-UGI
UseCRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ269E2
Plasmid#164721PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
UseCRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti myc-NL-GW
Plasmid#113454PurposeLentiviral NanoLuc Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviral and LuciferaseTagscmyc-NanoLucExpressionMammalianMutationPromoterhUbCAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB+_attB53
Plasmid#183614PurposeRMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUDP012
Plasmid#101167PurposeE. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265G2
Plasmid#164723PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-ziSpyMac(D10A)-UGI)
UseCRISPR; Gateway compatible a3a/y130f-ispymaccas9-…TagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ165
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEVA1
Plasmid#58953PurposeContains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.DepositorInsertVAR1u, VAR1 recoded for expression from the nucleus
UseYeast ecoli shuttle vectorTagspreCox4 mitochondrial matrix targeting signalExpressionYeastMutationSynthetic gene in standard genetic codePromoterADH1Available sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CCL5.Pro
Plasmid#79492PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoterDepositorInsertCCL5 Promoter (CCL5 Human, Synthetic)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCCL5 PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m
Plasmid#161522PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editingDepositorInsertTadA(wt)-TadA(7.10)-zSpCas9(D10A)
UseCRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR E2 HSPter-RPS5Ap-mito
Plasmid#163146PurposePlant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORFDepositorInsertA terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW-mCit-PA
Plasmid#113457PurposeLentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseLentiviralTagsmCitrine-ProteinAExpressionMammalianMutationPromoterhUbCAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only