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pCMV-myc3-HDM2

Plasmid

#20935

Depositor

Yue Xiong

Article

Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

Insert

Mdm2 p53 binding protein homolog (MDM2 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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AAVi U6_gRNA CMV_SadCas9-KRAB

Plasmid

#214609

Purpose

All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

Depositor

Ralf Gilsbach

Article

Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

Inserts

gRNA
SadCas9

Use

AAV

Tags

NP NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CMV and U6

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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lentiBE3-SpRY-Blast

Plasmid

#199303

Purpose

Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

FNLS-BE3-SpRY

Use

CRISPR and Lentiviral

Tags

2A tag

Expression

Mammalian

Mutation

Promoter

EFS

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-puro-PTPIP51(1-470)

Plasmid

#170536

Purpose

Expresses PTPIP51(1-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-mNeongreen-Etanercept

Plasmid

#182421

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-Etanercept (TNFRSF1B Human)

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-DNAseI

Plasmid

#182409

Purpose

Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DNase I (DNASE1 Bovine)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Article

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Insert

Akt1 (AKT1 Human)

Use

Tags

HA and flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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PRPF19

Plasmid

#155847

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF19 (PRPF19 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Plasmid

#180475

Purpose

Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

Zebrafish ubb promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTET2-RlucCP-3MS2

Plasmid

#198354

Purpose

Part of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTR

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Renilla Luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

pTET2

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-CD80-mGFP

Plasmid

#223591

Purpose

Lentiviral expression of human CD80 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-flag S127A YAP

Plasmid

#27370

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

S127A ( major phosphorylation site in YAP2)

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PABPC1

Plasmid

#155805

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PABPC1 (PABPC1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Myc-YAP

Plasmid

#33091

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV/MSV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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p13xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180480

Purpose

Inducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

13x QUAS-ZsGreen-P2A

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Dox-Akt

Plasmid

#207328

Purpose

PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

Depositor

Jared Toettcher

Article

Underhill et al Development. 2023 Aug 15;150(16):dev2016

Insert

rac protein kinase-alpha (AKT1 Human)

Use

Tags

myristoylation tag - BFP

Expression

Bacterial

Mutation

range: 129 - 480

Promoter

Tet Response Element

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJBEI-6409

Plasmid

#47048

Purpose

BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene

Use

Synthetic Biology; Bglbrick

Tags

Expression

Bacterial

Mutation

Promoter

PlacUV5

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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Lentiviral-His-tagged-PSPH

Plasmid

#134786

Purpose

Stable overexpression lentiviral vector of His tagged PSPH

Depositor

Seon-Young Kim

Article

Park et al Mol Cells. 2019 Aug 31;42(8):604-616. do

Insert

Phosphoserine phosphatase (PSPH Human)

Use

Lentiviral

Tags

His

Expression

Mutation

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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