Addgene

Showing: 2861 - 2880 of 4607 results

Results per page:

pcDNA3-HCMVgO-8his (Merlin)

Plasmid

#136454

Purpose

Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein O (UL74 Human betaherpesvirus 5)

Use

Tags

Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pSHDY*in_PpetE:BCD2:mVenus

Plasmid

#133970

Purpose

Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

mVenus

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-BaLgp160-QES.01

Plasmid

#100921

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations T49D;P1…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-BaLgp160-QES.02

Plasmid

#100923

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations T49D;P1…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-BaLgp140-8his

Plasmid

#100924

Purpose

Mammalian expression plasmid for soluble gp140 from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence and Purification tag after D6…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-BaLgp120-8his

Plasmid

#100925

Purpose

Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Q769gp120-8his

Plasmid

#100929

Purpose

Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-Q769gp160-QES.03

Plasmid

#100930

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations A200E;F…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Q842gp120-8his

Plasmid

#100932

Purpose

Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Use

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)

Use

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA24N-ligase-pprA

Plasmid

#87744

Purpose

IPTG-inducible expression of ligase-pprA fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis and codon optimized pprA (from Deinococcus …

Expression

Bacterial

Mutation

Promoter

T5-lac

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA24N-sso7d-ligase

Plasmid

#87745

Purpose

IPTG-inducible expression of sso7d-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis and codon optimized sso7d (Sulfolobus solfa…

Expression

Bacterial

Mutation

Promoter

T5-lac

Availability

Academic Institutions and Nonprofits only

Enlarge
p28-2iDMD-puro

Plasmid

#88895

Purpose

28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.

Depositor

Michele Calos

Article

Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

Inserts

Dystrophin
luciferase
puromycin resistance enzyme
mCherry

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-gp120(BaL)-CD4

Plasmid

#98957

Purpose

Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

HIV-1 (BaL) gp120

Use

Tags

CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

Plasmid

#99144

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEXm-GST-ATG14

Plasmid

#99329

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

ATG14 (ATG14 Human)

Use

Tags

GST-TEV tag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_dCas9-2xAM_hIRF-1

Plasmid

#92221

Purpose

Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Inserts

Sp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter

Use

CRISPR and Lentiviral

Tags

2xAM tag

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

CBh and U6

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-MH6-RspCas13d_RspCasWYL1

Plasmid

#108304

Purpose

Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Inserts

RspCas13d
RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Mutation

Promoter

lac

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3cH_pGAP_23*_pLAT1_Cas9

Plasmid

#104907

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
BB3cK_pGAP_23*_pTEF_Cas9

Plasmid

#104909

Purpose

hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge