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SGF29 (3MEU)
Plasmid#25317PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_6 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 115-293PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGF29 (3LX7)
Plasmid#25314PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29_3 (SGF29 Human)
UseTagsHisExpressionBacterialMutationContains amino acids 138-291PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
C6orf197
Plasmid#25258PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC6orf197:M178-R505 (TBC1D22B Human)
UseTagsHisExpressionBacterialMutationContains amino acids M178-R505PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PLXND1 (3H6N)
Plasmid#25187PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPLXND1:A1553-D1656 (PLXND1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids A1553-D1656PromoterAvailable sinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP-CDPK1 (3DXN)
Plasmid#25129PurposeBacterial expression for structure determination; may not be full ORFDepositorInsert541.m00134:MAC02T-C05:C40091
UseTagsHisExpressionBacterialMutationContains amino acids L72-Q339PromoterAvailable sinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE
Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralTagsExpressionMutationDR1 A>TPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE
Plasmid#138151PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR_BsmBI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-p3E-HyPer7
Plasmid#194289PurposeMultisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQVDepositorInsertHyper7
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-Ngn3PDX1MafA
Plasmid#114303PurposeConstitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptidesDepositorInsertNgn3 (NEUROG3 Human)
UseLentiviralTagsExpressionMutationNgn3, PDX1 and MafA proteins are expressed from a…PromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBT-PE2-PuroTK-pegRNA_GG
Plasmid#173222PurposepiggyBac transposon containing both PE2 and a pegRNA cloning site.DepositorInsertsPE2
PuroTK
pegRNA cloning cassette
UsePiggybacTagsExpressionMammalianMutationPromoterCMV, P2A, and hU6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2303
Plasmid#91068PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2312
Plasmid#91070PurposeModule B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRTagsExpressionMutationPromoterPvUbi1Available sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2112
Plasmid#91064PurposeModule B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoterPvUbi1Available sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2103
Plasmid#91061PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)
Plasmid#154824Purpose(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegansDepositorInsertPeft-3::wrmScarlet::tbb-2 UTR
UseTagsExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2615
Plasmid#91078PurposeModule B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertBsaI ccdb cassette for single gRNA spacer cloning
UseCRISPRTagsExpressionMutationPromoterAtU6Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-com-Ds
Plasmid#119077PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loopsDepositorInsertU6a:2xCom-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-MS2-Ds
Plasmid#119076PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loopsDepositorInsertU6a:2xMS2-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE
Plasmid#138145PurposeExpresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.DepositorInsertPspCas13b
UseLentiviralTagsNLSExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only
