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Plasmid#114040PurposeKinetochore localised FRB tagged with FLAGDepositorInsertMIS12 (MIS12 Human)
UseTagsFRB-FLAG-tagRFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB226
Plasmid#68617PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-BsaI-EGFP
Plasmid#123136PurposeGolden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertEGFP, mRFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromotercBAAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB236
Plasmid#68627PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJOG642
Plasmid#105406PurposeSynthetic biologyDepositorInsert3xHA tag fused to tagRFP-T CDS
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3105 (pSpy1C-PxylS2_ffluc)
Plasmid#218512Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPxylS2Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA279
Plasmid#216001PurposeFragmid fragment: (Selection) fluorescent reporterDepositorHas ServiceCloning Grade DNAInsertStart; TagRFP_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav-RA
Plasmid#209881PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1DepositorInsertddRFP A
UseTagsCAVIN1ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB560-NADK2
Plasmid#203760PurposeFor Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertNAD KINASE2
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK411.RR1
Plasmid#192644PurposeExpresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpRDepositorInsertBBa_J23119-RR1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB554
Plasmid#74884PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB555
Plasmid#74885PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-NLS
Plasmid#140900Purposemammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-NLS
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mito-RA
Plasmid#209866PurposeDimerization dependent fluorescent protein R anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddRFP A
UseTagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-RA
Plasmid#209872PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddRFP A
UseTagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev
Plasmid#118474PurposeddRFP-based single-color biosensor for monitoring ERK activity.DepositorInsertRAB-EKARev
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Perox-RA
Plasmid#209869PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3DepositorInsertddRFP A
UseTagsTargeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEC-red
Plasmid#196040PurposeAllows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate AssemblyDepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Grna cloningTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB454
Plasmid#74836PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only