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Plasmid#191678PurposePtwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarletDepositorInsertGCaMP6s
UseTagswScarletExpressionWormMutationPromoterPtwk-40Available sinceNov. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
PDZD7-TwinStrepII
Plasmid#83198PurposeExpression of tagged protein in mammalian cellsDepositorInsertPDZD7 (Pdzd7 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
UseTagsExpressionWormMutationPromotereft-3Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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FH-TET3-HxDmut-pEF
Plasmid#127896PurposeExpresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079ADepositorInsertTET3-HxDmut (TET3 Human)
UseTagsHA and FlagExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NINJ1
Plasmid#201179PurposeExpression of NINJ1 gene in mammalian cells by retroviral transductionDepositorInsertNinjurin 1 (Ninj1 Mouse)
UseRetroviralTagsFlagExpressionMutationPromoterMESVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-CXXCmut-pEF
Plasmid#127895PurposeExpresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAADepositorInsertTET3-CxxCmut (TET3 Human)
UseTagsHA and FlagExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR
Plasmid#129774PurposeMarker of microtubule dynamics in C. elegans hypodermisDepositorInsertsUseSynthetic Biology; GatewayTagsSynthetic Intron and mGFPExpressionWormMutationPromoterPcol-19Available sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre stuffer v3
Plasmid#158030Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJR2956
Plasmid#122533PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH4330
Plasmid#191700PurposePunc-17::GtACR2::wCherry unc-54 3' UTR C.elegans neuron expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPunc-17Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)
Plasmid#107137PurposeDominant negative PKA cGAL effector for C. elegansDepositorInsertkin-2a(G310D)::SL2::GFP
UseTagsExpressionWormMutationPromoter15xUAS-delta-pes-10(basal promoter)Available sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH4329
Plasmid#191679PurposePrgef-1-GtACR2::wCherry unc-54 3' UTR C.elegans Pan-neural expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPregf-1Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSVE Grb2 myc 49L
Plasmid#26085DepositorInsertGrb2 P49L (GRB2 Human)
UseTagsmycExpressionMammalianMutationP49LPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UBE3BΔIQ-HA-IRES-Puro
Plasmid#107350PurposeExpresses HA tagged UBE3B deletion of IQ domain in mammalian cells.DepositorInsertUBE3BdelIQ (UBE3B Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJR2955
Plasmid#122550PurposeExpression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemNeptune2.5
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH3666
Plasmid#191447PurposePacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFPDepositorInsertGCaMP6s
UseTagswCherryExpressionWormMutationPromoterPacr-2sAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfKitlgb
Plasmid#168200PurposeExpress zebrafish Kitlgb, mRNA synthesisDepositorInsertKitlgb (kitlgb Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationI85V, V222I, and D233N- please see depositor comm…PromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0146_Cq-U6-1_2xBbsI-gRNA
Plasmid#169238PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertCq-U6-1_2xBbsI-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039653Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only