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Plasmid#139442PurposeAPEX2-Lamin B1 expression plasmidDepositorInsertLMNB1 (LMNB1 Human)
UseTags3xGGGGS linker (between APEX2 and Lamin B1), APEX…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-NLS-Smad2
Plasmid#14931DepositorInsertNLS-Smad2 (SMAD2 Human)
UseTagsFlagExpressionMammalianMutationfull-length Smad2 is fused to a nuclear-localizat…PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-FLAG-Synaptojanin 1-145
Plasmid#22293DepositorInsertSynaptojanin 1_145 (SYNJ1 Human)
UseTagsFlag and GFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos MAL Flag
Plasmid#41554DepositorInsertMAL (TIRAP Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKK-FLAG-BirA-TEV
Plasmid#105770PurposeExpression of your protein of interest in fusion with FLAG-BirA. FLAG-tagged at the N-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsFLAG-BirA-TEVExpressionMammalianMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-Flag-h-SNRNP40
Plasmid#134254PurposeLentivector encoding Flag-tagged human SNRNP40DepositorInsertSNRP40 (SNRNP40 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Yap1 Y357F
Plasmid#18882DepositorInsertYap1 Y357F (YAP1 Human)
UseTagsFlagExpressionMammalianMutationY357FPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Synaptojanin 1-145
Plasmid#22291DepositorInsertSynaptojanin 1_145 (SYNJ1 Human)
UseTagsFlagExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-Fml1
Plasmid#193058PurposeBaculovirus expression of S.pombe Fml1 for recombinant protein productionDepositorInsertFml1 (fml1 Fission Yeast)
UseTags3xFlagExpressionInsectMutationPromoterpolyhedronAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 FLAG-SFRP2-Fc
Plasmid#115772PurposeExpression of the extracellular domain of SFRP2 with N-terminal FLAG tag and C-terminal hIgG tag.DepositorInsertSFRP2 (SFRP2 Human)
UseTagsFLAG and hIgG/FcExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND1
Plasmid#172648PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST TNRC6A
Plasmid#19883DepositorInsertTrinucleotide repeat containing 6A (TNRC6A Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 RpH-LAMP1-3xFLAG
Plasmid#163019Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAGExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK Smad2 deltaC Flag
Plasmid#12624DepositorInsertSmad2 deltaC (SMAD2 Human)
UseTagsflagExpressionMammalianMutationC-terminal truncated Smad2 (aa 1-424)PromoterAvailable sinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-human NPM1-C1
Plasmid#160693PurposeMammalian expression of FLAG-tagged NPM1 C1 deletion mutant lacking nucleic acid-binding domain (NBD).DepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationLacks NBD domainPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-CaMKIID-K43M
Plasmid#186830PurposeExpresses FLAG-CaMKIID-K43M in mammalian cellsDepositorInsertCamk2d (Camk2d Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Lysine 43 to MethioninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag-RagA(Q66L)
Plasmid#99716PurposeMammalian expression of Flag-RagA(Q66L)DepositorInsertRagA (RRAGA )
UseTagsFlagExpressionMutationQ66LPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (WT) FLAG GFP
Plasmid#164978PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorInsertFLCN (FLCN Human)
UseLentiviralTags1xFLAG-GFPExpressionMammalianMutationPromoterUBCAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt1W80A
Plasmid#64833PurposecDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drugDepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPoint mutation from Trp to Ala at position 80PromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK Smad3 deltaC Flag
Plasmid#12626DepositorInsertSmad3 (SMAD3 Human)
UseTagsflagExpressionMammalianMutationC-terminal truncated Smad3 (aa 1-382)PromoterAvailable sinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only