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Plasmid#141047PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9-L2
Plasmid#141040PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'GAC)
Plasmid#170127PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'GAC)
Plasmid#170129PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti_P2A_Blast
Plasmid#211365PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-dNme2Cas9
Plasmid#193190PurposeLentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)DepositorInsertdNme2Cas9
UseTagsNLS and NLS-3xHA-NLSExpressionMammalianMutationH588A, D16APromoterSFFVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170816PurposeLentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170817PurposeLentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-IDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G121C/G13A-nick sgRNA
Plasmid#214099PurposeLentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3DepositorInsertKRAS G12C or G13A nicking sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide-PolB1-puro
Plasmid#177146PurposeLentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassetteDepositorInsertPolBgRNA1 (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170813PurposeLentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170814PurposeLentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreenDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170815PurposeLentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
hU6-pegRNA-EF-1α-Cre Template
Plasmid#196036PurposeThe template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6 and EF-1-alphaAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
hU6-pegRNA-EFS-mScarlet Template
Plasmid#196037PurposeThe template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.DepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterhU6 and EFSAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190033Purposevector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13X
UseTagsExpressionMammalianMutationY672A,Y676APromoterCBh, SV40, hU6Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190034Purposevector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13d
UseTagsExpressionMammalianMutationA134V,A140V,A141V,A143VPromoterCBh, SV40, hU6Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP
Plasmid#112677PurposeAn AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent proteinDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertNuclear-localized floxed-mCherry EGFP
UseAAVTagsNuclear localization signalExpressionMutationPromoterEF1aAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-DNMT1
Plasmid#66818PurposedCas9 fused to BFP and the human DNMT1 catalytic domainDepositorInsertdCas9-BFP-DNMT1, catalytic domain (DNMT1 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only