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pCW57-GFP-2A-Cas9

Plasmid

#170805

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

Depositor

Morten Frodin

Article

Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CHEK1 C1.2 gRNA

Plasmid

#90626

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHEK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CHEK1 (Guide Designation C1.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pS848∙CsR

Plasmid

#199240

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Gm resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

PEM7

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJG80

Plasmid

#89282

Purpose

Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Insert

TaCas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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KIF2A D7.2 gRNA

Plasmid

#90720

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF2A (Guide Designation D7.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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KIF2A D8.2 gRNA

Plasmid

#90721

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF2A (Guide Designation D8.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA2 A11.2 gRNA

Plasmid

#90550

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A11.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-Cas9C

Plasmid

#80932

Purpose

Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTX209

Plasmid

#89269

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA01 and OsPDS-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Maize Ubiquitin1 promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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PB-EEA-g1-PGK-Puro

Plasmid

#102908

Purpose

PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-guide1-PGK-Puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i-Max-T2A-EGFP

Plasmid

#188268

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-Max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i-HiFi-T2A-EGFP

Plasmid

#188269

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-HiFi

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAS9id

Plasmid

#141251

Purpose

Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

Depositor

Adam Bertl

Article

Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

GAL1 (Cas9), pSNR52 (gRNA)

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGal-BE3

Plasmid

#172409

Purpose

Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

Depositor

Leonid Kruglyak

Article

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

GALL

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-gRNA

Plasmid

#53062

Purpose

Transcription of guide RNA (gRNA) in wheat cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

wheat U6 promoter - gRNA

Use

CRISPR; Plant expression

Tags

Expression

Mutation

Promoter

wheat U6

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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sgRNA 1 GAPDH

Plasmid

#83808

Purpose

Express Sg-1 sgRNA targeting GAPDH

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFH6

Plasmid

#86555

Purpose

Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

Depositor

Andreas Weber

Article

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

U6-26p::sgRNA scaffold

Use

CRISPR; Subcloning

Tags

Expression

Mutation

Promoter

Arabidopsis U6-26 promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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EZH2 E10.3 gRNA

Plasmid

#90685

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E10.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

Plasmid

#164040

Purpose

Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

sgTS5-sgTS6-sgTS7

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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