Addgene

We narrowed to 30,455 results for: CMV

Showing: 2841 - 2860 of 30455 results

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pCMV-luc-miR-122

Plasmid

#171557

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-122

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98H-pRc/CMV

Plasmid

#22044

Depositor

William Kaelin

Article

Ohh et al Mol Cell. 1998 Jun . 1(7):959-68.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 98 from Tyrosine to H…

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G469A

Plasmid

#131711

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

G469A

Promoter

CMV

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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Stat1 beta pRc/CMV

Plasmid

#8703

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 beta (STAT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2005

Availability

Academic Institutions and Nonprofits only
pRC/CMV-JAK1K908E-VSV

Plasmid

#139357

Purpose

Expression of kinase-dead JAK1-K908E protein

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

K908E

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-LMO-IRES-EGFP

Plasmid

#178309

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hNedd4 (C867A) DN

Plasmid

#24125

Depositor

Peter Howley

Insert

Nedd4 DN (NEDD4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pRc/CMV2 Dvl-2-Myc

Plasmid

#42194

Depositor

Shin-ichi Yanagawa, Robert Lefkowitz

Article

Lee et al J Biol Chem. 1999 Jul 23;274(30):21464-7

Insert

Dvl 2 (Dvl2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-hA3A-BE3-Y132D

Plasmid

#113425

Purpose

Expresses hA3A-BE3-Y132D in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

hAPOBEC3A_Y132D

Promoter

CMV

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-deltaR8.2(A14C,E45C)

Plasmid

#79047

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pHR'CMVGFP_hRIF1(406-2446)IREShygro

Plasmid

#23135

Depositor

Lifeng Xu

Article

Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

Insert

RIF1 deletion construct (RIF1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

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miniCMV eGFP hPGK BSD

Plasmid

#188519

Purpose

Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV/MASC_(GLuc)_TOP1

Plasmid

#68439

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-BE4max-BlastR

Plasmid

#152991

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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hPDI1-18-508_C400S-pFLAG-CMV1

Plasmid

#31384

Depositor

David Ron

Article

Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

Insert

hPDI1 (P4HB Human)

Use

Tags

FLAG M1

Expression

Mammalian

Mutation

C400S

Promoter

Available since

May 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1 H83L H86L

Plasmid

#12211

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

PBD (p21-binding domain) mutant: H83L, H86L (see …

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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CMV-FLAG-Core-R (pMO29)

Plasmid

#24480

Depositor

Melanie Ott

Article

Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

Insert

Core

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pTYF-mCMV/GfaABC1D-EGFP

Plasmid

#19976

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 . 8():49.

Insert

mCMVGfaABC1D-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
Nxn-1 beta Flag/pCMV

Plasmid

#197339

Purpose

Plasmid expressing an antigen targeting Neurexin 1beta

Depositor

Thomas Südhof

Insert

Neurexin 1beta (Nrxn1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-hMcl-1(S159A)

Plasmid

#25393

Depositor

Roger Davis

Insert

MCL1 (MCL1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 159 to Alanine

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only